大蒜素对人胃癌细胞 SGC-7901及BGC-823G2/M 期的阻滞调节机制
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柳 星, 马 锐, 陈 威, 白雪蕾, 袁 媛
大蒜素;SGC-7901;BGC-823;G2,M期阻滞;CDC2;CyclinB,大蒜素对人胃癌细胞SGC-7901及BGC-823G2,M期的阻滞调节机制,柳星,马锐,陈威,白雪蕾,袁媛,柳星
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柳星, 马锐, 陈威, 白雪蕾, 袁媛, 中国医科大学附属第一医院肿瘤研究所第三研究室 辽宁省沈阳市 110001
柳星, 女, 1970-04-21生, 辽宁省沈阳市人, 汉族, 2002年中国医科大学肿瘤学硕士生, 副主任医师, 主要从事肿瘤临床及基础研究.
十五国家科技攻关项目, No. 2004BA703B04-02
通讯作者: 袁媛, 110001, 辽宁省沈阳市和平区南京北街155号, 中国医科大学附属第一医院肿瘤研究所第三研究室. yyuan@mail.cmu.edu.cn
电话：024-23256666-6153 传真：024-22703576
收稿日期: 2005-08-29 接受日期: 2005-09-06
Inhibition of Allicin on G2/M phase of human gastric cell line SGC-7901 and BGC-823
Xing Liu, Rui Ma, Wei Chen, Xue-Lei Bai, Yuan Yuan
Xing Liu, Rui Ma, Wei Chen, Xue-Lei Bai, Yuan Yuan, the Third Laboratory of the Cancer Institute, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Supported by the National Science and Technology Research and Development Program of China during the 10th Five-Year Plan Period, No. 2004BA703B04-02
Correspondence to: Professor Yuan Yuan, the Third Laboratory of Cancer Institute, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. yyuan@mail.cmu.edu.cn
Received: 2005-08-29 Accepted: 2005-09-06
Abstract
AIM: To explore the inhibitory effect of Allicin on the G2/M phase of human gastric cell line SGC-7901 and BGC-823 and its mechanism.
METHODS: The proliferation inhibition rate and IC50 of Allicin (72 h) to SGC-7901 and BGC-823 cells were examined by MTT assay. The cycles of the cells were measured by flow cytometry when IC50 concentration of Allicin was adopted. The expression of CDC2 and CyclinB protein were detected by immunohistochemistry.
RESULTS: Allicin inhibited the proliferation of SGC-7901 (IC50 = 23 mg/L) and BGC-823 (IC50 = 35 mg/L) cells and the inhibition rate was elevated with the increase of Allicin concentration. After treatment with IC50 concentration of Allicin, the percentage of G0/G1 phase cells was decreased, but that of G2/M phase ones was significantly increased for both SGC-7901 (24, 48 h vs control: 26 ......
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