TRAIL受体在胰腺癌中的表达及意义
胰腺肿瘤,,胰腺肿瘤·肿瘤坏死因子相关凋亡诱导配体·肿瘤坏死因子相关凋亡配体受体,1材料和方法,2结果,3讨论,参考文献
【摘要】目的：研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)受体在胰腺癌中的表达及意义。方法：应用半定量RTPCR，检测TRAILR mRNA在胰腺癌组织，正常胰腺组织及胰腺癌细胞系ASPC1、Canpan2中的表达。结果：死亡受体DR4、DR5在所有胰腺癌组织、正常胰腺组织及胰腺癌细胞系中均有表达，诱骗受体DcR1、DcR2在所有正常胰腺组织及细胞系中均有表达。死亡受体DR4、DR5在胰腺癌组织中有较高的表达，而在正常胰腺组织中呈中低水平表达(P<0.01)。胰腺癌细胞系中死亡受体DR4、DR5呈高水平表达，而诱骗受体DcR1、DcR2仅呈中低水平表达。结论：TRAIL受体在胰腺癌普遍表达，并存在受体类型的表达差异；死亡受体在胰腺癌中高表达，可能在TRAIL诱导胰腺癌细胞凋亡的机制中发挥重要的作用。【关键词】胰腺肿瘤·肿瘤坏死因子相关凋亡诱导配体·肿瘤坏死因子相关凋亡配体受体
Expression and significance of tumor necrosis factorrelated apoptosisinducing ligand recepors in pancreatic carcinoma
GONG Wei,LIANG Fei,LI Zhanyuan,ZENG Qingdong,LIU Jun,WANG Lei,Lü Bin,ZHOU Yong
1Center of organ Transplantation,3Department of General Surgery,Shandong Provincial Hospital(Jinan 250021,China)
2Department of General Surgery,Qilu Hospital of Shandong University(Jinan 250012,China)
【ABSTRACT】Objective:To investigate the expression and significance of tumor necrosis factorrelated apoptosisinducing ligand(TRAIL) recepors in pancreaitc carcinoma.Methods:The expression of TRAILR mRNA was assayed by semiquantitive RTPCR in 30 pancretic carcinoma tissues,18 normal pancreatic tissues and 2 pancreatic cancer cell lines of ASPC1、Canpan2.Results:The expression of death receptor 4(DR4) and death receptor 5(DR5) was detected in all pancreatic carcinoma tissues,normal pancreatic tissues and pancreatic cancer cell lines,decoy receptor (DcR1) and decoy receptor 2(DcR2) were expressed in normal pancreatic tissues and pancreatic cell lines.The expression of DR4 and DR5 in the pancreatic carcinoma tissues was significantly higher than that in the normal pancreatic tissues(P0.01).Conclusion:TRAILR expression is prevalent in pancreatic tissues with different receptor types existing.High death receptor expression may play an important role in TRAIL inducing pancreatic carcinoma apoptosis. ......
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