大鼠____

大鼠腹膜伤害性刺激cfos在中枢神经系统表达的实验研究

http://www.100md.com 《新疆医科大学学报》 2005年第5期

大鼠,,Fos,蛋白；腹膜伤害性刺激；中枢神经系统；免疫组化,1材料与方法,2结果,3讨论

     摘要：目的: 研究大鼠腹膜伤害性刺激cfos在中枢神经系统的表达及分布。方法 :将1%的乙酸注入大鼠腹膜腔造成腹痛模型,用免疫组化ABC法检测中枢神经系统内cfos阳性神经元的分布。结果：A组(对照组)T6~L3脊髓后角、臂旁核、室旁核有少量cfos表达，实验组与对照组比较Cfos蛋白表达差异有统计学意义(P<0.05)，提示cfos阳性神经元呈双侧分布，主要分布于T6~L3脊髓后角、延髓孤束核、臂旁核、蓝斑核、下丘脑、室旁核、视上核、杏仁核。结论：T6~L3脊髓后角、延髓孤束核、臂旁核、蓝斑核、下丘脑、室旁核、视上核、杏仁核与大鼠腹膜伤害性刺激造成的腹痛有关，本研究为进一步进行神经生物学研究镇痛机制提供了实验依据。

    关键词： Fos 蛋白；腹膜伤害性刺激；中枢神经系统；免疫组化

    Empirical study of the rat peritoneal noxious stimulation inducing expression of cfos in central nervous system

    ZHANG Jie, GAN Ziming, CHENG Shenguo

    (Department of Human Anatomy, Preclinical Medicine College，Xinjiang Medical University, Urumqi 830054, China)

    Abstract :objective: To study expression and distribution of the rat peritoneal noxious stimulation inducing Fos protein(Fos) in central nervorus system. Method: 1% acetic acid was injected into rat peritoneal cavity to make the abdomino pain model. The localization of cfos positive neurons was detected by means of immunohistochemistry (ABC method) in central nervous system. Result: cfos of A control group is little expression in central nervorus system. Experiment groups compared with controlling group, the result has statistical value (P<0.05). The cfos positive neurons are distributed in both sides of CNS mainly located within T6~L3 posterior horn of spinal cord, nucleus tractus solitarii, parabrachial nucleus, locus cerulei nucleus; dorsal nucleus of midraph. Hypothalamus, paraventricular nucleus, supraoptic nucleus and amygdaloid nucleus. Conclusion: Above mentioned nuclei might involve in rat abdomino pain which is caused by peritoneal noxious stimulation, which offers groundwork of experiment for further studying killing pain with nervous biology. ......

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