摘要：目的 探讨心肌缺血预处理(MIP)对未成熟心肌bcl-2、bax、fas蛋白表达的影响。方法 采用兔Langendorff模型，24只幼兔随机分为3组，对照组(C,n=8)：仅灌注KH液180min，然后取标本；缺血/再灌注组(I/R, n=8)：灌注20min后，停灌45min，复灌120min；心肌缺血预处理组(MIP, n=8)，灌注20min，反复2次停灌5min，复灌5min，然后重复I/R组缺血/再灌方法。以凋亡细胞原位标记、琼脂糖凝胶电泳检测DNA片段梯及bcl-2、bax、fas蛋白表达作为检查指标。结果 琼脂糖凝胶电泳检测DNA片段梯：MIP组与I/R组比较，光密度明显减弱； MIP组与I/R组比较，bcl-2表达明显增多，bax、fas表达明显减少。结论 MIP通过影响bcl-2、bax、fas基因蛋白的表达减少心肌细胞凋亡。

    关键词： 缺血预处理；心肌；细胞凋亡；基因蛋白

    The Effects of Myocardial Ischemic Preconditioning on Immature Myocardial Cell Apoptosis

    SUN Zhong-dong, CHI Yi-fan, YANG Tie-nan ,HOU Wen-ming ,NIU Zhao-zhuo,TANG Zi-long

    (Department of Cardiovascular Surgery, Qingdao municipal Hospital ,Medical College of Qingdao University ,Shandong Qingdao 266011, China)

    Abstract: OBJECTIVE To investigate the effects of myocardial ischemic preconditioning (MIP) on immature myocardial cell apoptosis and expression of bcl-2,bax,fas protein .METHODS Isolated working rabbit heart model was used in this study, 24 rabbits (aged 14～21 days) were divided into 3 groups (n=8) . Control group was only perfused with Krebs-Henseleit (KH) 180 minutes. Ischemia/reperfusion(I/R) group was perfused 20 minutes ,and then kept under ischemia 45 minutes, followed by reperfustion 120 minutes. Myocardial ischemic preconditioning (MIP) group was perfused 20 minutes ,and then repeatly ischemia 5 minutes followed reperfusion 5 minutes 2 times,finnaly reperfused 120 minutes. Such indexes, including the TUNEL, nucleosomal ladder of DNA fragments, and the expression of bcl-2, bax,and fas gene were detected.RESULTS MIP group there was reduction in TUNEL cell apoptosis, ladder of DNA fragments,expression of bax and fas gene,and increase in expression of bcl-2 gene.CONCLUSION This study demonstrated that MIP decrease immature myocardial cell apoptosis by affecting the expression of bcl-2, bax,and fas gene . ......