肺癌患者化疗前后外周血淋巴细胞MRP1的表达
流式细胞技,,肺癌；流式细胞技术；外周血淋巴细胞；多药耐药相关蛋白1,1资料与方法,2结果,3讨论,参考文献：
摘要：目的：探讨肺癌患者化疗前后外周血淋巴细胞(PBL)多药耐药相关蛋白1(MRP1)的表达，寻找检测肺癌多药耐药的新途径。方法：应用流式细胞免疫学方法，检测10例健康人和30例肺癌患者化疗前后PBL的MRP1表达，并比较了肺癌患者化疗前后PBL的MRP1表达；根据化疗疗效评价标准将肺癌患者分为有效组和无效组，比较了两组患者化疗前后PBL的MRP1表达。结果：健康人PBL的MRP1表达为(2.01±0.68)%；肺癌患者化疗前与化疗后PBL的MRP1表达相比差异有统计学意义(t=-4.051, P<0.05)；有效组化疗前后PBL的MRP1表达差值与无效组的比较差异无统计学意义(t=-1.145, P>0.05)。结论：(1)流式细胞免疫法检测PBL的MRP1表达具有简便、可操作性；(2)化疗后肺癌患者PBL的MRP1表达与化疗前相比有增高趋势,提示存在化疗后的继发耐药；(3)PBL的MRP1表达与化疗疗效无关，尚不能预测化疗疗效。关键词: 肺癌；流式细胞技术；外周血淋巴细胞；多药耐药相关蛋白1
Study on MRP1 expression in PBL from patients with lung cancer before and after chemotherapy
QU Yanli， JIANG Jiayu， SHAN Li
(Second Department of Medical Oncology, Cancer Hospital, Xinjiang Medical University,Urumqi 830011, China)
Abstract: Objective: To explore MRP1 expression in PBL from patients with lung cancer before and after chemotherapy,and to probe a new method for detecting multidrug resistance in lung cancer. Methods: Using flow cytometry to detect the expression of MRP1 in PBL from 10 healthy persons and 30 cases with untreated lung cancer before and after chemotherapy,and to compare MRP1 expression in PBL before with after chemotherapy; All patients were divided into efficacy and inefficacy based on curative effect, and to compare MRP1 expression before and after chemotherapy in both groups. Results: The mean expression of MRP1 in PBL from healthy persons was (2.01±0.68)%；the differences of expressions of MRP1 in PBL before and after chemotherapy were significant (t=-4.051, P<0.05). The differences in both groups before and after chemotherapy were not significant (t=-1.145, P>0.05). Conclusions: (1) The method to detect MRP1 expression in PBL with flow cytometry is feasible ......
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