RNA干扰_____RNA干扰；_辣椒素受体1；

应用RNAi技术调节VR1基因表达

http://www.100md.com 《新疆医科大学学报》 2005年第3期

RNA干扰,,RNA干扰；,辣椒素受体1；,疼痛,1材料与方法,2结果,3讨论,参考文献：

     摘要: 目的：通过调节辣椒素受体1(VR1)基因表达，对VR1基因的功能及疼痛的基因治疗进行基础研究。方法：应用RNA干扰(RNAi)技术干扰VR1基因在猴肾Cos7细胞系中的表达。结果：应用RNAi技术特异性抑制了VR1基因在Cos7细胞中的表达。随着小片段干扰性RNA(siRNA)浓度的增加，VR1蛋白的表达量逐渐减少。结论：应用RNAi技术可抑制VR1基因的表达，为开发更有效、安全、合理的镇痛药提供实验依据。

    关键词：RNA干扰；辣椒素受体1；疼痛

    Regulation of VR1 gene expression by RNAi technique

    Kurexi, Jens Kurreck， Arnold Grünweller, et al

    (Department of Biochemistry and Moleculebiology, Xinjiang Medical University,Urumqi 830054, China)Abstract: Objective: By regulation of VR1 gene expression, fundamentally investigate function of VR1gene and pain's gene therapy. Methods: To apply RNAi technique to regulate the expression of VR1gene in Cos7cell. Results: Expression of VR1 gene in Cos7 cell is specially inhibited by siRNA'S concentration increasing and the VR1 protein's expression are decreasing. Conclusions: Expression of VR1 gene in Cos7 cell is specially inhibited by using RNAi Technique, it will provide the theoretical evidence for development of more effective, safe, reasonable analgesic. ......

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