一种新型阿尔茨海默病动物模型的建立
新型,,阿尔茨海默病；动物模型；AlCl3；鞘内注射,1材料和方法,2结果,3讨论,参考文献:
摘要: 目的：探讨AlCl3鞘内注射的神经毒性，并对应用此方法建立阿尔茨海默病(AD)的动物模型进行初步评价。方法：将12个月龄SD大鼠随机分为4组。行为学实验结束后，常规石蜡切片，用ABC法进行βAP、βAPP和tau免疫组织化学染色，HE染色观察颗粒空泡变性(GVD)变化，Bielschowsky染色观察神经原纤维缠结(NFTs)。结果：模型组海马与皮质βAPP、βAP、tau阳性神经元细胞数高于对照组同区域数值(P<0.05)。大脑顶叶和海马切片神经细胞数目减少，模型组GVD细胞数有明显增加；可见神经纤维缠结样病理改变。结论：AlCl3鞘内注射具有明确的在体神经毒性作用，提示该AD动物模型简便、实用，并能较好地模拟AD行为和病理表现。关键词：阿尔茨海默病；动物模型；AlCl3；鞘内注射
Establishment of novel animal model with Alzheimer′s disease
YANG Yong, MA Long, ZHOU Xiaohui, et al
(Department of Toxicology, College of Public Health, Xinjiang Medical University, Urumqi 830054, China)
Abstract: Objective: To establish the Alzheimer′s disease (AD) model through the methodpretreatment with aluminium chloride by intrathecal injection to investigate the neuropathology in the AD models. Methods: 12monthold SD rats were divided into four groups randomly. Model groups rats administrated with aluminum chloride by intrathecal injection (AlCl3:0.5%、1.0%、1.5%；IT for 5 days). To make paraffin section after behaviour test. Silver staining by a modified Bielschowsky method for NFTs and ABC immunocytochemical method for SPs and histochemistry of hippocampi for GVD. Results: Aluminum could result in the decrease of neurons in cerebral cortex and hippocampus.The number of GVD cells in model group increased significantly. βAP, tau and βAPP expressed in the cytoplasm of both neurons and glial cells in cerebrum. The results showed that the frequency of electric shock was increased, incubation period was decreased. Conclusion: βAP、tau andβAPP expression was increased significantly in ADanimalmodel′s cerebrum,also can form NFT. The changes were some similar to behavior characterizations and the pathological manifestation of human Alzheimer′s disease. This method may be a simple and promising animal models in AD. ......
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