腰交感神经切除术_

大鼠直、结肠炎CLS的镇痛作用及COX2与GFAP在CNS表达的形态学观察

http://www.100md.com 《新疆医科大学学报》 2005年第4期

腰交感神经切除术,,腰交感神经切除术(LS)；COX2；GFAP；CNS,；直结肠炎,1材料与方法,2结果,3讨论,参考文献：

     摘要：目的：研究化学性腰交感神经切除术(CLS)对直结肠慢性刺激性炎症和扩张性伤害性刺激(CRD)的中枢保护作用，为临床医学提供基础研究资料。方法：Wistar 大鼠54只，随机分为6组，每组9只，按二因素析因实验设置实验组，另外设立实验对照组和空白对照组。利用直、结肠慢性刺激性炎性致痛模型，切除模型大鼠的腰交感神经后，运用机械扩张刺激观察大鼠行为变化。用 Miampamba 评分方法改良后疼痛四级评分标准评分，重复3次取均值记录结果。取间脑、脑桥、延髓、颈段(C5~8)、胸段(T3~6)、腰段(L1~3)、骶段(S2~4)脊髓连续切片后，延髓和脊髓用COX2，间脑、脑桥用胶质纤维酸性蛋白(GFAP)免疫组化染色，显微镜下观察相应核团,计数阳性细胞数。结果：LS无论作为预防性手术还是治疗性手术均能使中枢内GFAP和COX2表达阳性的细胞数下降(P<0.05)。NST,C5~8中间带和T3~6中间带外侧核不是直结肠慢性炎症刺激和CRD扩张性伤害刺激上传通路中的中继神经元(P>0.05)。一、二、三、四级反射各组比较直结肠扩张刺激阈值与手术顺序无关而与手术有关(P<0.05)。假手术不影响痛觉阈值(P>0.05)，而手术后痛觉阈值明显上调(P<0.05)。结论：化学性腰交感神经切除术(CLS)在直结肠慢性炎症性刺激和CRD伤害刺激过程中能有效的保护中枢神经系统。腰交感神经是可能的直结肠伤害性刺激上传的传入神经。CLS的中枢保护作用与手术时间顺序无关。

    关键词：腰交感神经切除术(LS)；COX2；GFAP；CNS ；直结肠炎

    The effects of lumbar sympathectomy in coloproctitis and express of COX2 and GFAP in central nervous system

    XU Xiao, CHEN Shengguo, GAN Zimin, et al

    (Department of Human Anatomy, Xinjiang Medical University, Urumqi 830054, China)

    Abstract: Objective: To study the protective effect of chemical lumbar sympathectomy on central nervous system of coloproctitis's model rats under colonrectum distension harm and to offer basic medical reference for clinic use. Methods: Fiftyfour Wistar rats are divided into 6 groups randomly,each group contain 9 rats. According to 2 factorial experiment design, experiment groups, experiment contrast group and control group were set up. Chemical lumbar sympathectomies were performed on coloproctitis hyperalgesia model and colonrectum distension (CRD) was used as harm disturbing. Miampamba's standard of evaluation for visceral pain which was divide pain into 4 grade was used to observe their behavior. repeat test for three times then record the mean value of visceromotor threshold (VMT). Finally the rats's brain and spinal cord were take out and paraffin section were made, the medulla oblongata and spinal cord are using COX2, the diencephalon and pons are using GFAP.The positive cells were counted by microscope. Results: Coloproctitis hyperalgesia and colonrectum distension (CRD) can be smoothed by chemical lumbar sympathectomy in central nervous system(P<0.05). Coloproctitis hyperalgesia and colonrectum distension (CRD) are not be delivered by NST, C5~8,T3~6 (P>0.05). By Ⅰto Ⅳ reflection comparing, the visceromotor threshold (VMT) didn't combined with the sequence but the operation itself(P<0.05). False operation didn't influence the visceromotor threshold (VMT) (P>0.05). However operation can promote the visceromotor threshold (VMT) (P<0.05). Conclusion: Chemical lumbar sympathectomy can efficiently protect CNS in the process of coloproctitis's model rat under colonrectum distension harm. It can reduce the positive cell in CNS after CRD. The chemical lumbar sympathetic is a probably way to CNS for colonalgia and CRD. The CNS which was protected by CLS have nothing to do with sequence of operation, but the operation itself. ......

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