【摘要】 目的 旨在探讨血管内皮生长因子(VEGF)在肺癌组织中的表达及其临床价值以及VEGF与肿瘤生长、转移及预后的关系。方法 对38例肺癌组织以免疫组织化学法研究VEGF在肿瘤组织的胞内分布及其表达;同时以ELISA法测定临床上肺癌、良性肺病患者及正常对照血清中的VEGF水平。结果 肺癌组织中VEGF位于胞质和胞膜，呈棕黄色颗粒状过度表达，Ⅲ期、Ⅳ期肺癌和伴远处转移者阳性率为90%，肺癌患者血清中VEGF水平显著高于正常对照(P<0.01)，但在良恶性肺病组间未见明显差异，经抗感染药物治疗后，良性肺病患者血清VEGF水平明显下降。结论 资料提示肺癌发生和发展过程中VEGF呈过度表达，检测患者血清VEGF可以作为肺癌诊断和预后的有用指标。

    关键词 血管内皮生长因子 肺癌 免疫组化 酶联免疫吸附分析 诊断

    Abnormal expression of vascular endothelial growth factor and

    its diagnosis and prognosis values in patients with lung cancer

    Feng Jian，Wu Xinhua，Ni Songshi，et al.

    Affiliated Hospital of Nantong Medical College，Jiangsu226001.

    【Abstract】 Objective To explore changes and clinical values of vascular endothelial growth factor(VEGF)in diagnosis and prognosis of patients with lung cancers.Methods The VEGF cellular distributions and expression in38specimens of patients with lung cancer were investigated with immunohistochemistry assay.The levels of VEGF in sera of patients with lung cancer，benign lung diseases and healthy controls were detected through ELISA method.Results The VEGF positive stain

    was76%in38cases of lung cancer specimens.The89%rate of VEGF stain was found for clinical stageⅢcases and92%for stageⅣlung cancers.The significantly higher expression of VEGF was eviˉdenced in patients with lymph node metastasis(84%) ......