胃癌组织中法尼基转移酶基因表达与临床病理特点及H-ras突变的关系
http://www.100md.com
顾华丽, 田字彬, 王其新
胃癌;法尼基转移酶;H-ras基因;突变;聚合酶链反应,顾华丽,王其新,田字彬,通讯作者:,电话:,收稿日期:,接受日期:,Re
参见附件(448kb)。
顾华丽, 王其新, 青岛大学医学院附属医院急诊内科 山东省青岛市 266003
田字彬, 青岛大学医学院附属医院消化内科 山东省青岛市 266003
顾华丽, 女, 1973-11-13生, 江苏省丹阳人, 汉族, 2005年青岛大学医学院内科学消化系病硕士, 主治医师.
通讯作者: 顾华丽, 266003, 山东省青岛市江苏路16号, 青岛大学医学院附属医院急诊内科. qdguhuali@163.com
电话: 0532-82911345
收稿日期: 2005-08-03 接受日期: 2005-08-26
Relations farnesyltransferase beta-subunit mRNA expression with clinicopathological features and H-ras mutation in gastric cancer tissues
Hua-Li Gu, Zi-Bin Tian, Qi-Xin Wang
Hua-Li Gu, Qi-Xin Wang, Department of Emergency for Internal Medicine, the Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China
Zi-Bin Tian, Department of Gastroenterology, the Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China
Correspondence to: Dr. Hua-Li Gu, Department of Emergency for Internal Medicine, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province,China. qdguhuali@163.com
Received: 2005-08-03 Accepted: 2005-08-26
Abstract
AIM: To investigate the expression of farnesyltransferase(FTase) beta-subunit mRNA in gastric cancer tissues and its relations with the clinicopathological features and point mutation in codon 12 of H-ras gene.
METHODS: Specimens were collected from 43 cases of gastric cancer and their corresponding normal tissues. The expression of FTase beta-subunit mRNA was investigated by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The point mutation in codon 12 of H-ras gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Wilcoxon Signed-Rank Test was used to analyze the difference of the matching data of FTase beta-subunit mRNA expression between gastric cancer and normal tissues. Multiple linear regression analysis was used to explore the relations of FTase activity with the clinicopathological features and H-ras mutation in gastric cancer.
RESULTS: The mean level of FTase beta-subunit mRNA expression were significantly higher in gastric cancer tissues than that in the corresponding normal ones(0.89 ± 0.48 vs 0.69 ± 0.40, Z = 2.469, P = 0.014). Of the 43 cases, the mutation of H-ras 12 codon was found in 1 case of cancer tissue(1/43, 2.3%), and no mutation appeared in the all the normal tissues. The expression of FTase beta-subunit mRNA was not related to the age, tumor location, histological differentiation, lymph node metastasis and H-ras 12 codon mutation. But the female patients with signetring cell carcinoma had higher expression of FTase beta-subunit mRNA.
CONCLUSION: The expression of FTase beta-subunit mRNA is up-regulated in gastric cancer. The point mutation in codon 12 of H-ras gene is not related to the expression of FTase beta-subunit mRNA.
Key Words: Gastric cancer; Farnesyltransferase; H-ras gene; Mutation; Polymerase chain reaction ......
顾华丽, 王其新, 青岛大学医学院附属医院急诊内科 山东省青岛市 266003
田字彬, 青岛大学医学院附属医院消化内科 山东省青岛市 266003
顾华丽, 女, 1973-11-13生, 江苏省丹阳人, 汉族, 2005年青岛大学医学院内科学消化系病硕士, 主治医师.
通讯作者: 顾华丽, 266003, 山东省青岛市江苏路16号, 青岛大学医学院附属医院急诊内科. qdguhuali@163.com
电话: 0532-82911345
收稿日期: 2005-08-03 接受日期: 2005-08-26
Relations farnesyltransferase beta-subunit mRNA expression with clinicopathological features and H-ras mutation in gastric cancer tissues
Hua-Li Gu, Zi-Bin Tian, Qi-Xin Wang
Hua-Li Gu, Qi-Xin Wang, Department of Emergency for Internal Medicine, the Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China
Zi-Bin Tian, Department of Gastroenterology, the Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China
Correspondence to: Dr. Hua-Li Gu, Department of Emergency for Internal Medicine, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province,China. qdguhuali@163.com
Received: 2005-08-03 Accepted: 2005-08-26
Abstract
AIM: To investigate the expression of farnesyltransferase(FTase) beta-subunit mRNA in gastric cancer tissues and its relations with the clinicopathological features and point mutation in codon 12 of H-ras gene.
METHODS: Specimens were collected from 43 cases of gastric cancer and their corresponding normal tissues. The expression of FTase beta-subunit mRNA was investigated by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The point mutation in codon 12 of H-ras gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Wilcoxon Signed-Rank Test was used to analyze the difference of the matching data of FTase beta-subunit mRNA expression between gastric cancer and normal tissues. Multiple linear regression analysis was used to explore the relations of FTase activity with the clinicopathological features and H-ras mutation in gastric cancer.
RESULTS: The mean level of FTase beta-subunit mRNA expression were significantly higher in gastric cancer tissues than that in the corresponding normal ones(0.89 ± 0.48 vs 0.69 ± 0.40, Z = 2.469, P = 0.014). Of the 43 cases, the mutation of H-ras 12 codon was found in 1 case of cancer tissue(1/43, 2.3%), and no mutation appeared in the all the normal tissues. The expression of FTase beta-subunit mRNA was not related to the age, tumor location, histological differentiation, lymph node metastasis and H-ras 12 codon mutation. But the female patients with signetring cell carcinoma had higher expression of FTase beta-subunit mRNA.
CONCLUSION: The expression of FTase beta-subunit mRNA is up-regulated in gastric cancer. The point mutation in codon 12 of H-ras gene is not related to the expression of FTase beta-subunit mRNA.
Key Words: Gastric cancer; Farnesyltransferase; H-ras gene; Mutation; Polymerase chain reaction ......
您现在查看是摘要介绍页，详见PDF附件(448kb)。