苦玄参__(中华医药)

HPLC法测定苦玄参中苦玄参苷IA的含量

http://www.100md.com 《中华医药杂志》 2005年第11期

苦玄参,,苦玄参；苦玄参苷IA；含量测定；高效液相色谱法,1仪器与试药,2含量测定方法与结果,3结果讨论,【参考文献】

     【摘要】目的建立苦玄参药材Picria felterrae Lour.的含量测定方法。方法 RP-HPLC法，采用C18ODS2柱(5μm，4.6×250mm)，乙腈-0.3%磷酸(34∶66)为流动相,流速为1ml/min，检测波长为264nm。结果苦玄参苷IA进样量在0.42～2.10μg的范围内呈良好的线性关系，r=0.9999，平均回收率为100.4%，RSD=1.64%(n=6)。结论该法操作简便、准确、专属性强，可作为苦玄参药材中苦玄参苷IA的质量控制方法。

    【关键词】苦玄参；苦玄参苷IA；含量测定；高效液相色谱法

    Determination of piefeltarraeninIA in picria felterrae Lour by HPLC

    CHEN Yong，ZHEN Hanshen， LIU Jing，et al.

    Faculty of Pharmacy, Guangxi University of TCM，Nanning 530001,China

    【Abstract】 Objective To establish a method for the determination in picria fel-terrae Lour.Methods RP-HPLC.The piefeltarraeninIA was separated on a C18ODS2 column(5μm,4.6×250mm) and detected at 264nm and 1ml/min,using acetonitrile-0.3%phosphoric acid (34∶66) as the mobile phase. Results PiefeltarraeninIA in picria felterrae Lour was baseline separated and determined.The linearity rangs was 0.48μg～2.10μg, correlation coefficient was 0.9999.The average recovery was 100.4%(RSD=1.64%, n=6). Conclusion The method is simple, accurate and with strong specificity and can be used for quantify the main constituient piefeltarraeninIA in picria felterrae Lour. ......

百拇医药网 http://www.100md.com/html/DirDu/2005/11/23/80/47/71.htm

您现在查看是摘要页，全文长 6873 字符。