J-HNP-1 多肽的重组及体外抗菌作用研究

http://www.100md.com 刘贤华, 白晓东, 仝青英,张韶峰

 重组PCR;抗菌肽;J链;a-防御素-1刘贤华,白晓东,仝青英,张韶峰.J-HNP-1多肽的重组及体外抗菌作用研究. 世界华人消化杂志2005;13(22)2640-2644

第1页

    参见附件(987KB，5页)。

     刘贤华,仝青英, 张韶峰，中国人民武装警察部队总医院科训科中心实验室 北京市 100039

    白晓东, 中国人民武装警察部队总医院烧伤整形外科 北京市 100039

    刘贤华, 女, 1971-04-19生, 湖北省洪湖市人, 汉族, 1999年重庆市第三军医大学预防医学系本科毕业, 2005年重庆市第三军医大学硕士研究生毕业, 实验师, 主要从事创伤后感染预防和治疗的研究.

    国家自然科学基金资助项目, No. 30100197

    通讯作者：刘贤华, 100039, 北京市海淀区永定路69号, 北京市武警总医院科训科中心实验室. liuxianhua99student@sina.com

    电话: 010-88276752 传真：010-68211327

    收稿日期：2005-07-25 接受日期：2005-08-26

    Preliminary study on recombination ofJ-HNP-1 and its antibacterial effect in vitro

    Xian-Hua Liu, Xiao-Dong Bai,Qing-Yin Tong, Shao-Feng Zhang

    Xian-Hua Liu, Qing-YinTong, Shao-Feng Zhang, CentralLabortary, General Hospital of Chinese People’s Armed Police Force,Beijing 100039, China

    Xiao-Dong Bai, Departmentof Burn and Plastic Surgery, General Hospital of Chinese People’s ArmedPolice Force, Beijing 100039, China

    Supported by NationalNatural Science Foundation of China, No. 30100197

    Correspondence to: Dr.Xian-Hua Liu, Central Laboratory, General Hospital of Chinese People’sArmed Police Force, Beijing 100039, China. liuxianhua99student@sina.com

    Received: 2005-07-25Accepted: 2005-08-26

    Abstract

    

    AIM: Toconstruct the recombinant of J chain and human neutrophil peptide-1(HNP-1) gene, and to express it in the mammalian cell system.

    METHODS: The J chainand HNP-1 cDNA were ampli-fied from the different plasmids by polymerasechain reaction (PCR), respectively, then the two kinds of cDNA fragmentswere recombined to obtain J-HNP-1 gene by recombinant PCR. After theJ-HNP-1 cDNA fragment was inserted into the mammalian express vectorpcDNA3.1(-)/Myc-HisC, the recombinant vector was transfected to the COS-7cells by lipid transfection. The expression of J-HNP-1 mRNA and proteinwere detected by reverse transcription RCP (RT-PCR) and Western-blot,respectively. The antibacterial activities of cellular soluble protein andculture supernatant were examined in vitro.

     ......