小鼠暴发性肝衰竭肝细胞凋亡形态学及其调控机制
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王玉梅, 冯国和, 窦晓光, 刘德刚
一氧化氮;Fas抗原;Bcl-2;暴发性肝衰竭王玉梅,冯国和,窦晓光,刘德刚.小鼠暴发性肝衰竭肝细胞凋亡形态学及其调控机制.世界华人消化杂志2005;13(22)2658-2662,小鼠暴发性肝衰竭肝
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王玉梅, 冯国和, 窦晓光，中国医科大学附属第二医院感染科辽宁省沈阳市 110004
刘德刚，鞍山市曙光医院医疗科辽宁省鞍山市 114031
王玉梅, 女, 1970-02-26生, 山西省大同市人, 2002年中国医科大学博士,副教授, 主要从事病毒性肝炎发病机制的研究.
通讯作者：王玉梅,110004, 辽宁省沈阳市, 中国医科大学附属第二医院感染科.wangym7002@yahoo.com
电话: 024-83956961 传真: 024-62124785
收稿日期：2005-08-03 接受日期：2005-08-26
Morphology and regulatory mechanism of hepatocyte apoptosis in experimental fulminant hepatic failure
Yu-Mei Wang, Guo-He Feng, Xiao-Guang Dou, De-Gang Liu
Yu-Mei Wang, Guo-He Feng, Xiao-Guang Dou, Department of Infectious Diseases, the Second Affiliated Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
De-Gang Liu, Department of Medicare, Shuguang Hospital, Anshan 114031, Liaoning Province, China
Correspondence to:Yu-Mei Wang, Department of Infectious Diseases, the Second Affiliated Hospital of China Medical University, Shenyang 110004, Liaoning Province,China. wangym7002@yahoo.com
Received:2005-08-03 Accepted:2005-08-26
Abstract
AIM:To study the morphological changes and the regulation of nitric oxide (NO), Fasand Bcl-2 on hepatocyte apoptosis in mouse model of experimental fulminant hepatic failure (FHF)..
METHODS:Mouse model of experimental FHF was established by combination of lipopolysaccharide (LPS) and D-galactosamin (D-GalN). The expression of Fas and Bcl-2 in the liver tissues was tested by immunohistochemistry. The level of serum NO and iNOS mRNA expression in liver were tested by nitrate reductase method and reverse transcription polymerase chain reaction (RT-PCR), respectively. The hepatocyte apoptosis was examined by TUNEL method. In addition, the changes of the above items were observed after pretreatment with L-NMMA, an inhibitor of iNOS.
RESULTS:The level of serum NO and expression of iNOS mRNA in the liver tissues were increased at 2 h in model group, reaching the peak at 4 h ......
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