d氨基酮戊酸-光动力疗法诱导SW480 细胞钙调素和突变型 p53的表达变化
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郑江华, 时德, 陈祖林, 梁 平
结肠癌;d氨基酮戊酸;光动力疗法;SW480;钙调素;突变型p53;基因表达郑江华,时德,陈祖林,梁平.d氨基酮戊酸-光动力疗法诱导SW480细胞钙调素和突变型p53的表达变化.世界华人消化杂志200
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郑江华, 时德,重庆医科大学附属第一医院血管外科 重庆市 400016
陈祖林, 梁平, 第三军医大学新桥医院普外科 重庆市 400037
国家自然科学基金资助项目, No. 30271481
通讯作者:陈祖林, 400037, 重庆市，第三军医大学新桥医院普外科. chenzulin@163.com
电话: 023-68774405 传真: 023-68774605
收稿日期: 2005-09-02 接受日期: 2005-09-30
Expression of calmodulin and mutantp53 in SW480 cells in response to delta-aminolevulinic acid-basedphotodynamic therapy
Jiang-Hua Zheng, De Shi, Zu-Lin Chen, Ping Liang
Jiang-Hua Zheng, De Shi, Department of Vascular Surgery, the First Affiliated Hospital of Chongqing MedicalUniversity, Chongqing 400016, China
Zu-Lin Chen, Ping Liang, Department of General Surgery, Xinqiao Hospital of the Third Military MedicalUniversity, Chongqing 400037, China
Supported by National Natural Science Foundation of China, No. 30271481
Correspondence to: Zu-Lin Chen, Department of General Surgery, Xinqiao Hospital of the Third MilitaryMedical University, Chongqing 400037, China. chenzulin@163.com
Received: 2005-09-02 Accepted: 2005-09-30
Abstract
AIM:To investigate the expression of calmodulin (CaM) and mutant p53 in human colon carcinoma SW480 cell lines after induced by delta-aminolevulinic acid (ALA)-based photodynamic therapy (PDT).
METHODS:SW480 cells were divided into control, laser light, ALA, and ALA-PDT group. The expression of CaM3 and mutant p53 in SW480 cells were detected by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: The relative contents of CaM3 in the contr-ol, light, ALA group, and ALA-PDT 30, 60, and 90 min group were 3.97±0.29, 4.28±0.39, 4.51±0.44, 12.60±1.84, 11.39±1.13, and 12.77±1.35, respectively. The expression of CaM3 in the ALA-PDT 30-90 min group was significantly increased in comparison with that in the control, light, and ALA group (P <0.001). The relati-ve expression of p53 gene in the control, light, ALA group, and ALA-PDT 30, 60, and 90 min group were 1 ......
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