慢性乙醇中毒所致大鼠肝损伤和肝细胞凋亡
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伏建峰, 史清海, 岳新华, 张东辉
乙醇;肝损伤;凋亡;TUNEL,伏建峰,史清海,岳新华,张东辉,伏建峰,通讯作者:,Liverinjuryandhepatocyteapoptosisi
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伏建峰, 史清海, 兰州军区乌鲁木齐总医院检验科 新疆维吾尔族自治区乌鲁木齐市 830000
岳新华, 兰州军区乌鲁木齐总医院病理科 新疆维吾尔族自治区乌鲁木齐市 830000
张东辉, 兰州军区乌鲁木齐总医院动物实验科 新疆维吾尔族自治区乌鲁木齐市 830000
伏建峰, 男, 1967生, 云南昆明人, 汉族, 2003年新疆医科大学硕士, 副主任技师, 主要从事临床生化研究.
通讯作者: 伏建峰, 830000, 新疆维吾尔族自治区乌鲁木齐市友好北路41号, 兰州军区乌鲁木齐总医院检验科. gs29xy@msn.com
电话: 0991-4992101-352
收稿日期: 2005-09-21 接受日期: 2005-09-30
Liver injury and hepatocyte apoptosis induced by chronic alcoholic intoxication in rats
Jian-Feng Fu, Qing-Hai Shi, Xin-Hua Yue, Dong-Hui Zhang
Jian-Feng Fu, Qing-Hai Shi, Department of Clinical Laboratory, Urumqi General Hospital of Lanzhou Military Command, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
Xin-Hua Yue, Department of Pathology, Urumqi General Hospital of Lanzhou Military Command, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
Dong-Hui Zhang, Laboratory For Animal Science, Urumqi General Hospital of Lanzhou Military Command, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
Correspondence to: Jian-Feng Fu, Department of Clinical Labor-atory, Urumqi General Hospital of Lanzhou Military Command, 41 Youhao North Road, Urumqi 830000, Xinjiang Uygur Autonomous Region, China. gs29xy@msn.com
Received: 2005-09-21 Accepted: 2005-09-30
Abstract
AIM: To investigate the role of hepatocyte apoptosis in the pathogenesis of alcohol-induced liver diseases (ALD) in rats.
METHODS: The rat model of liver injury was induced by combination of drinking and gastric irrigation of ethanol. The morphological changes of the liver were observed by routine HE staining under light microscope. The hepatocyte apoptosis was examined by TUNEL, and the levels of serum alanine aminotransferase (ALT) and aspartate transaminase (AST) were detected by the rate method.
RESULTS: At the end of the 5th week, the light and moderate steatosis appeared in ethanol-treated rat livers, the proportion of fatty degeneration was 40% (8/20); At the end of the 10th week, the proportion was increased to 85%(17/20), and the morphological changes of alcoholic hepatitis (AH) were found in 45%(9/20) rats ......
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