DNCB 所致结肠炎小鼠血清中细胞因子的活性
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余万桂, 张恒文, 胡承明, 张道明, 梅 林
溃疡性结肠炎;细胞因子;2,4-二硝基氯苯;小鼠,DNCB所致结肠炎小鼠血清中细胞因子的活性,余万桂,张恒文,胡承明,张道明,梅林,余万桂,通讯作者,Activityofcytokinesind
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余万桂, 张恒文, 胡承明, 张道明, 长江大学医学院机能学部 湖北省荆州市 434000
梅林, 北京大学医学部生理与病理生理学系 北京市 100083
余万桂, 女, 1970-07-17生, 湖北省荆州市人, 汉族, 2001年华中科技大学生命科学与技术学院硕士, 副教授, 主要从事消化道神经免疫调节研究.
重庆市科学基金应用基础研究项目, No. 8108
通讯作者：余万桂, 434000, 湖北省荆州市江汉路55号, 长江大学医学院机能学部. wanguiy@yahoo.com.cn
电话：0716-8067988 传真：0716-8067647
收稿日期：2005-10-25 接受日期：2005-10-31
Activity of cytokines in dinitrochlorobenzene-induced colitis in mice
Wan-Gui Yu, Heng-Wen Zhang, Cheng-Ming Hu, Dao-Ming Zhang, Lin Mei
Wan-Gui Yu, Heng-Wen Zhang, Cheng-Ming Hu, Dao-Ming Zhang, Department of Functional Science, College of Medical Science, Yangtze University, Jingzhou 434000,Hubei Province, China
Lin Mei, Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing 100083, China
Correspondence to: Wan-Gui Yu, Department of Functional Science, College of Medical Science, Yangtze University, 55 Hanjiang Road, Jingzhou 434000, Hubei Province, China. wanguiy@yahoo.com.cn
Received: 2005-10-25 Accepted: 2005-10-31
Abstract
AIM: To observe the changes of serum pro-inflammatory and anti-inflammatory cytokine activities in mice with experimental colitis.
METHODS: A total of 33 BALB/c mice were randomly divided into control (n = 15) and experiment group(n = 18). After sensitized by 2, 4-dinitrochlorobenzene (DNCB) smeared on the abdominal skin, the mice in the experiment group were challenged with DNCB enema, while those in the control group were treated with ethanol. The body weight, stool viscosity and hematochezia were observed and accumulated as disease active index (DAI) score; the colon pathological score was achieved by macropathology and HE staining of section prepared for microscopy. The activity of serum IL-1, TNFa, IL-6, IL-4 and IL-10 was determined.
RESULTS: The percentage of diarrhea was 59% in the experiment mice 24 h after DNCB treatment, and serious weight loss was found, 20% mice died ......
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