【摘要】 目的 将荧光金(flurogold,FG)注射于SD大鼠上丘，观察到被FG逆行标记的视网膜神经节细胞(retinal ganglion cells,RGC)，以及SD大鼠RGC的数目及分布。方法 用3%FG双上丘注射，逆行标记RGC。5天后做视网膜定向铺片，在荧光显微镜下距离视乳头中心上下左右各2mm拍摄照片。利用计算机图像分析系统做RGC计数。结果 右眼RGC单位面积数量472.9±54.13，左眼RGC单位面积数量471.5±52.54。双眼RGC单位面积数量相比差异无显著性(P>0.05)。视网膜血管自视盘发出呈放射状，血管走行区无标记细胞。距视盘2mm始RGC分布较致密，视网膜周边部细胞分布较为稀疏。结论 用荧光金逆行标记上丘方法来标记RGC，是实验条件下研究RGC数量动态变化的可靠、有效方法。

    【关键词】 视网膜神经节细胞;荧光金

    Retinal ganglion cells retrograde labelled by injection of fluorogold

    LI Yue-hua，MA Ke，Xu Liang.

    Department of Ophthalmology,Beijing Chaoyang Hospital,Beijing 100020，China

    【Abstract】 Objective Retinal ganglion cells (RGC) were retrograde labeled by injection of 3% fluorogold into both side of superior colliculus to observe the numbers and distribution of RGC .Four photographs were taken from every quadrant of the retina 2mm from the center of optic disc. Retinal ganglion cells were quantitatively analyzed by computer.Methods To identify RGC,we applied the 3%fluorogold to the superior colliculi.The retinal were examined through a fluorescence microscope after 5 days,four photographs were taken from every quadrant of the retina 2mm from the center of optic disc. Retinal ganglion cells were quantitatively analyzed by computer.Results The numbers of RGC were not significantly different between two groups. The numbers of right eye was 472.9±54.13.The numbers of left eye was 471.5±52.54.Conclusion The method that retinal ganglion cells (RGC) retrograde labeled by injection of fluorogold is reliable and effective. ......