【摘要】 目的 建立肺炎清口服液的质量标准。方法 采用TLC法对本品中麻黄、苦参、丹参、前胡、栀子进行定性鉴别。采用RP-HPLC法，测定了方中主药黄芩中主要有效成分黄芩苷的含量。用ODS C18柱，流动相为甲醇-水-磷酸(53∶47∶0.0282)，检测波长278nm。结果 在薄层色谱中检出麻黄、苦参、丹参、前胡、栀子；色谱斑点清晰，阴性对照无干扰；黄芩苷在进样量0.408～2.040μg范围内进样量与峰面积积分值呈良好的线性关系，回归方程为Y=3.29×107X-1.85×105，r=0.9999。平均加样回收率为100.49%(RSD=1.7%，n=5)，肺炎清口服液中黄芩苷平均含量为5.042mg/ml。结论 所建立的方法可靠，结果稳定，重现性好，简便可行，为肺炎清口服液质量控制提供了方法。

    【关键词】 肺炎清口服液；薄层色谱法；高效液相色谱法；黄芩；黄芩苷

    Studies on quality standard of Fei-Yanqing oral liquid

    FENG Han-ge，WU Wen-ru.

    Hubei Provincial TCM Hospital， Wuhan 430061，China

    【Abstract】 Objective To establish a quality standard of Fei-Yanqing oral liquid.Methods Fructus gardeniae， Herba ephedrae， Radix peucedani， Radix salviae miltiorrhizae， Radix sophorae flavescentis were identified by thin-layer chromatography， and baicalin in Scutellaria baicalensis Georgi， was determined by RP-HPLC. An ODS C18 column was used. The mobile phase was methanol-water-phosphoric acid (53∶47∶0.0282). Detection wavelength was 278nm. Results The TLC spots were developed fairly clear， and the blank test showed no interference; A good linearity was showed at the concentration range of 0.408～2.040μg of baicalin ......