大黄多糖对溃疡性结肠炎小鼠结肠上皮细胞和外周血中性粒细胞凋亡的影响
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王志鹏, 张 蓉, 刘 莉, 梅其炳, 刘琳娜
大黄多糖;凋亡;溃疡性结肠炎;上皮细胞;中性粒细胞,王志鹏,张蓉,刘莉,梅其炳,刘琳娜,王志鹏,通讯作者:,Effectsofr
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王志鹏, 张蓉, 刘莉, 梅其炳, 刘琳娜, 第四军医大学药学系药理学教研室 陕西省西安市 710032
王志鹏, 1977-06-03生, 黑龙江省阿城市人, 汉族, 第四军医大学药学系药理学教研室博士生, 主要从事中药药理学研究和新药安全性评价工作.
国家自然科学基金资助项目, No. 30300450
通讯作者: 梅其炳，710032，陕西省西安市，第四军医大学药学系药理学教研室. qbmei@fmmu.edu.cn
电话: 029-83374552 传真：029-83374552
收稿日期: 2005-10-25 接受日期: 2005-10-31
Effects of rhubarb polysacchrides on apoptosis of colonic epithelial cells and peripheral blood polymorphonuclear neutrophils in mice with ulcerative colitis
Zhi-Peng Wang, Rong Zhang, Li Liu, Qi-Bing Mei, Lin-Na Liu
Zhi-Peng Wang, Rong Zhang, Li Liu, Qi-Bing Mei, Lin-Na Liu, Department of Pharmacology, School of Pharmacy, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Supported by National Natural Science Foundation of China, No.30300450
Correspondence to: Professor Qi-Bing Mei, Department of Pharmacology, School of Pharmacy, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. qbmei@fmmu.edu.cn
Received: 2005-10-25 Accepted: 2005-10-31
Abstract
AIM: To investigate the effects of rhubarb polysac-chrides (RP) on the apoptosis of colonic epithelial cells and peripheral blood polymorphonuclear neutrophils (PMN) in mice with ulcerative colitis, and to explore the mechanisms of RP in the treatment of ulcerative colitis.
METHODS: A mouse mode1 of ulcerative colitis was induced with 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) by enema. The mice were divided into three groups: nomal control, model, and RP treatment (400 mg/kg) group. TUNEL method was used to investigate the apoptosis of cells in colon, and the expression of Fas and FasL protein were measured by Western-blot. The level of Caspase 3 expression was detected by immunohistochemistry. Meanwhile, flow cytometry was used to examine the apoptosis of PMN.
RESULTS: The apoptosis level of colonic epithelial cells was dramatically higher in the model mice than that in the normal controls, while the rate of PMN apoptosis was significantly lower in the former (40 ......
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