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奥曲肽对肝星状细胞收缩的影响
http://www.100md.com 尹凤荣, 张晓岚, 王 川
奥曲肽;内皮素-1;肝星状细胞;胞质游离钙,尹凤荣,张晓岚,王川,通讯作者:,Effectofoctreotideoncontractionofrathepatics
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     尹凤荣, 张晓岚, 河北医科大学第二医院消化内科 河北省石家庄市 050000

    王川, 河北医科大学药理教研室 河北省石家庄市 050000

    河北省卫生厅资助项目, No. 2004110

    通讯作者: 张晓岚, 050000, 河北省石家庄市和平西路215号, 河北医科大学第二医院消化内科. xiaolanzh@126.com

    电话: 0311-87222951

    收稿日期: 2005-10-25 接受日期: 2005-10-31

    Effect of octreotide on contraction of rat hepatic stellate cell

    Feng-Rong Yin, Xiao-Lan Zhang, Chuan Wang

    Feng-Rong Yin, Xiao-Lan Zhang, Department of Gastroenterology, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China

    Chuan Wang, Department of Pharmacology, Hebei Medical University, Shijiazhuang 050000, Hebei Province, China

    Supported by the Fund from Department of Health of Hebei Province, No. 2004110

    Correspondence to: Dr. Xiao-Lan Zhang, Department of Gastroenterology, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. xiaolanzh@126.com

    Received: 2005-10-25 Accepted: 2005-10-31

    Abstract

    AIM: To investigate the effects of octreotide (OCT) on the contraction of endothelin-1(ET-1)-stimulated hepatic stellate cell (HSC), and to explore the changes of intracellular free calcium concentration ([Ca2+]i).

    METHODS: The changes of fluorescence degree, cellular area, and the [Ca2+]i that induced by OCT and ET-1 were observed by Fluo-3/AM method under confocal laser scanning microscope.

    RESULTS: Low concentration of OCT (2.5 mg/L) had no significant effect on the contraction or the [Ca2+]i in HSC. However, OCT at concentrations of 5.0 mg/L and 10.0 mg/L significantly reduced the [Ca2+]i immediately with the rate of 36.59% and 39.13% at 10 min (P < 0.05), respectively, but it had little effect on the contraction of HSC. ET-1 (10–8 mol/L) caused a transitory marked increase of [Ca2+]i in HSC, and a significant HSC contraction. OCT at concentrations of 5.0 mg/L and 10.0 mg/L inhibited the ET-1-induced [Ca2+]i increase and cell contraction in HSC (P < 0.05).

    CONCLUSION: OCT can reduce the concentration of [Ca2+]i immediately, but have little effect on the contraction of HSC. OCT can inhibit the ET-1-induced [Ca2+]i increase and cell contraction in HSC.

    Key Words: Octreotide; Endothelin-1; Hepatic stellate cell; Intracellular free calcium

    Yin FR, Zhang XL, Wang C. Effect of octreotide on contrac-tion of rat hepatic stellate cell. Shijie Huaren Xiaohua Zazhi 2006;14(1):79-82

    摘要

    目的:探讨奥曲肽(OCT)对内皮素-1(ET-1)刺激的肝星状细胞(HSC)收缩的影响, 以及此过程中胞质游离钙([Ca2+]i)的变化.

    方法:应用体外HSC培养技术, 借助激光共聚焦显微镜(LSCM), 采用第三代钙荧光探针Fluo-3/AM观察OCT、ET-1介导的HSC [Ca2+]i荧光强度、细胞面积及最大长径的变化.

    结果:2.5 mg/L OCT对HSC内Ca2+浓度及收缩无显著影响; 5.0 mg/L、10.0 mg/L组OCT能够即刻引起HSC内Ca2+浓度显著下降, 10 min时下降率分别为36 ......

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