关键词: 脑损伤;代谢性谷氨酸受体;基因表达;a-甲基-4-羧基苯氨基乙酸

    摘 要:目的 研究大鼠加速性弥漫性脑损伤后大脑皮层代谢性谷氨酸受体1a(mGluR

    1a )的基因和蛋白表达变化与其拮抗剂a-甲基-4-羧基苯氨基乙酸(MCPG)的作用. 方法 SD大鼠165只随机分为mGluR1a 变化和MCPG作用2组.每组又分为不同的亚组.采用Marmarou大鼠加速性弥漫性脑损伤模型.在伤后不同时间大鼠断头取脑进行免疫组化，RT-PCR技术和病理学研究. 结果 伤后1h大脑皮层mGluR1a 的表达开始增加，24h达高峰(13.9±2.3) HP-1 ，P<0.01.在伤后7d，MCPG治疗可使损伤神经元数量明显减少(4.22±1.63) HP-1 ，P<0.05. 结论 mGluR1a 参与外伤后的神经元损伤，其拮抗剂MCPG可能对脑损伤有治疗作用.

    Gene expression of metabotropic glutamate receptor1a after brain injury and efficacy of its antagonist MCPG

    HE Yuan-Dong，FEI Zhou，ZHANG Xiang，HE Xiao-Sheng，LI Shu-He，LIANG Jing-Wen

    Institute of Neurosurgery of Chinese PLA，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:head injury;mGluR;gene expression;MCPG

    Abstract:AIM To study the change of metabotropic gluta-mate receptor1a(mGluR

    1a )expression in a rodent model of impact acceleration diffuse brain injury and the effect of its antagonist a-methyl-4-carboxyphenylglycine(MCPG).METHODS 165male SD rats were randomized into two groups:Changes of mGluR1a and effect of MCPG.Each group was redivided into different subgroups.After Marmarouou's rodent model of impact acceleration diffuse brain injury ......