【摘要】 目的 探讨去甲硫氨酸(Met)环境是否提高化疗药物对胃癌细胞的杀伤作用。方法 将30例新鲜人胃癌组织制成单细胞悬液，分别培养于Met-Hcy+和Met+Hcy-培养基中，并在不同培养基中分别加入5-FU、DDP化疗药物，用MTT比色法检测各组细胞的抑制程度。结果 Met-Hcy+培养基分别与5-FU、DDP联合时，可显著提高化疗药物对胃癌细胞的杀伤作用。结论 Met-Hcy+培养基联合各化疗药物，可提高对胃癌细胞的杀伤作用。

    【关键词】 甲硫氨酸依赖；胃癌原代细胞；化疗

    The effect of methionine deprivation with chemotherapy to human primary gastric cancer cells

    ZHOU Lin-bin,LI Yun-wu,XU Ning,et al.

    Department of Surgery,Shanghai Ninth Hospital,Shanghai 200011,China

    【Abstract】 Objective To study the effect of methionine dependence in combination with chemotherapeutical agents on human primary gastric cancer cells.Methods 30 fresh gastric cancer samples were managed to single cell suspensions and then were cultured in Met-Hcy+ and Met+Hcy- medium separately,then 5-FU,DDP were added,the inhibition rate of tumor cells in different culture media was examined by microcytotoxicity(MTT) assay.Results Methionine dependence in combination with chemotherapy enhanced obviously the killing capacity of each chemotherapeutical agents on human primary gastric cancer cells.Conclusion The combined application of Met-Hcy+ medium and different chemotherapeutical agents could enhance the antitumor effect of chemotherapy on human primary gastric cancer cells. ......