关键词: 共刺激分子;骨肉瘤;转染

    摘 要:目的 为了研究共刺激分子B7增强针对大鼠骨肉瘤的特异性细胞免疫反应，首先需获得大鼠B7分子的cDNA克隆. 方法 应用反转录PCR的方法，从大鼠脾细胞mRNA中扩增特异性片段. 结果 经测序表明，所获得的cDNA克隆与已经发表的B7-1和B7-2的cDNA序列完全一致，并将其表达载体转染UMR-106细胞系. 结论 成功获得大鼠B7共刺激分子的cDNA克隆，其转染的UMR-106大鼠骨肉瘤细胞可用于免疫诱导研究.

    Cloning of rat B7cDNA and transfecting it into os┐teosarcoma cell line

    WU Yi-Chuan，HAO Xin-Bao，FAN Qing-Yu，YIN Jian-Ning，QIU Xiu-Chun，ZHANG Dian-Zhong

    Orthopedics Oncology Institute of Chinese PLA，Tangdu Hospital，Fourth Military Medical Univer-sity，Xi'an710038，China

    Keywords:co-stimulatory molecular;osteosarcoma;trans-fection

    Abstract:AIM In order to evaluate the effect of co-stimula-tor B7in tumor specific T cell activation ......