关键词: 胰岛素;脑缺血;Bcl-2;凋亡

    中图号:R659.1

    摘 要:目的 观察胰岛素对全脑缺血后海马CA1区神经元凋亡及Bcl-2蛋白表达的影响，探讨胰岛素对全脑缺血后海马CA1区神经元产生中枢直接保护作用的机制. 方法 利用4-VO法制作大鼠全脑缺血模型.造成脑缺血15min后行再灌注，于再灌注后即刻经脑室注入1U胰岛素，利用免疫组化及原位标记法分别于全脑缺血后1和3d观察海马CA1区Bcl-2蛋白表达及神经元凋亡的情况. 结果 全脑缺血后1d，缺血组及胰岛素治疗组鼠海马CA1区未见原位标记阳性细胞.治疗组鼠海马CA1区可见呈阳性表达的Bcl-2蛋白，而缺血组鼠海马CA1区Bcl-2蛋白的表达则呈阴性.全脑缺血后3d，缺血组鼠海马CA1区Bcl-2蛋白的表达仍呈阴性，海马CA1区原位标记阳性细胞计数为143.5±11.6.治疗组鼠海马CA1区Bcl-2蛋白呈阳性表达，海马CA1区原位标记阳性细胞计数为75.6±6.7. 结论 全脑缺血后脑室内注入胰岛素可促进海马CA1区Bcl-2蛋白表达，减少神经元的凋亡，这可能是其对全脑缺血后海马CA1区神经元产生中枢直接保护作用的主要机制之一.

    Effect of intraventricular administration of insulin on expression of Bcl┐2protein and the apoptosis of neuron in rat hippocampal CA1region

    LI Bing，ZHANG Xiang，FEI Zhou，ZHANG Zhi-Wen，WANG Yan-Gang，ZHANG Ge，JIANG Xiao-Fan，WU Jing-Wen，LIANG Jing-Wen

    Institute of Neurosurgery of Chinese PLA，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:insulin;brain ischemia;Bcl-2;apoptosis

    Abstract:AIM To investigate the mechanism of neuropro-tective effect of insulin on hippocamal CA1region，the ex-pression of Bcl-2protein and the apoptosis of neuron in rat hippocampal CA1region following global brain ischemia were observed.METHODS Four-vessel occlusion was used to es-tablish the rat global brain ischemic model.After15min brain ischemia ......