http://www.100md.com 刘春英, 王丽杰, 孙 梅, 赵恂, 胡 月, 赵
Change of prostaglandin E2 and effect ofplatelet activating factor antagonist on prostaglandin E2 inendotoxin-induced acute gastric mucosal injury in young rats
Chun-Ying Liu, Li-Jie Wang, Mei Sun,Xun Zhao, Yue Hu, Ya-Juan Zhao, Jun Li
Chun-Ying Liu, Li-Jie Wang, Mei Sun,Yue Hu, Ya-Juan Zhao, Jun Li, Department of Pediatrics, the SecondClincial College of China Medical University, Shenyang 110004, LiaoningProvince, China
Xun Zhao, the Second Department of Experimental Technique Center,China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to: Dr. Mei Sun, Department of Pediatrics, theSecond Clincial College of China Medical University, Shenyang 110004,Liaoning Province, China. firstname.lastname@example.org
Received: 2005-10-25 Accepted: 2005-10-28
AIM:To investigate the protective effect of prostaglandin E2 (PGE2) in endotoxin-induced acute gastric mucosal injury in young rats.
METHODS:Eighteen-day old Wistar rats were randomly divided into normal control, model, platelet activating factor (PAF) antagonist prevention and treatment groups. The model of endotoxemia of young rats was established by intraperitoneal injection of endotoxin (5 mg/kg E coli O55: B5 lipopolysaccharide, LPS). The rats in PAF prevention and treatment group were administered with PAF antagonist 0.5 h before and after modeling, respectively. The animals were killed 1.5, 3, 6, 24, 48, and 72 h after LPS injection. The pathological changes of gastric mucosa were observed by hematoxylin-eosin (HE) staining. The content of PGE2 was measured by radioimmunoassay method.
RESULTS:The pathological changes of gastric mucosa were significant 6 h after LPS injection. Erosion, bleeding and necrosis of gastric mucosa were observed. Swelled epithelial cells and developing degeneration were also observed. However, no remarkable changes occurred in both PAF antagonist groups. In model group, the level of PGE2 in gastric mucosa were significantly lower than that in the control (134.5 ± 9.3 g/L vs 245.1 ± 8.9 ?g/L, P < 0.01) 6 h after LPS injection. In PAF antagonist prevention and treatment groups, the levels of PGE2 were markedly higher than those in the model and control group (304.4 ± 15.0, 315.9 ± 43.7 g/L vs 134.5 ± 9.3, 245.1 ± 8.9 g/L, P < 0.01 or P < 0.05) 6 h after LPS injection.
CONCLUSION: The level of PGE2 in gastric mucosa is significantly decreased in endotoxemia, but it can be improved by PAF antagonist ......