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PGE2在内毒素诱导的幼鼠急性胃黏膜损伤中的变化及PAF受体拮抗剂对其影响
http://www.100md.com 刘春英, 王丽杰, 孙 梅, 赵恂, 胡 月, 赵
胃黏膜损伤;内毒素;前列腺素E2;血小板活化因子;PAF受体拮抗剂刘春英,王丽杰,孙梅,赵恂,胡月,赵雅娟,李军.PGE2在内毒素诱导的幼鼠急性胃黏膜损伤中的变化及PAF受体拮抗剂对其影响.世界华人消
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     刘春英,王丽杰,孙梅,胡月,赵雅娟,李军,中国医科大学第二临床学院儿科辽宁省沈阳市110004

    赵恂,中国医科大学实验技术中心二部辽宁省沈阳市110001

    刘春英,女,1963-03-17, 副主任医师,2003年中国医科大学博士在读,目前主要从事儿科危重症胃肠功能障研究

    通讯作者:孙梅,110004, 辽宁省沈阳市和平区三好街36号,中国医科大学第二临床学院儿科.wlj682002@yahoo.com.cn

    电话:024-23307620 传真:024-83955509

    收稿日期:2005-10-25 接受日期:2005-10-28

    Change of prostaglandin E2 and effect ofplatelet activating factor antagonist on prostaglandin E2 inendotoxin-induced acute gastric mucosal injury in young rats

    
Chun-Ying Liu, Li-Jie Wang, Mei Sun,Xun Zhao, Yue Hu, Ya-Juan Zhao, Jun Li

    Chun-Ying Liu, Li-Jie Wang, Mei Sun,Yue Hu, Ya-Juan Zhao, Jun Li, Department of Pediatrics, the SecondClincial College of China Medical University, Shenyang 110004, LiaoningProvince, China

    Xun Zhao, the Second Department of Experimental Technique Center,China Medical University, Shenyang 110001, Liaoning Province, China

    Correspondence to: Dr. Mei Sun, Department of Pediatrics, theSecond Clincial College of China Medical University, Shenyang 110004,Liaoning Province, China. wlj682002@yahoo.com.cn

    Received: 2005-10-25 Accepted: 2005-10-28

    Abstract

    AIM:To investigate the protective effect of prostaglandin E2 (PGE2) in endotoxin-induced acute gastric mucosal injury in young rats.

    METHODS:Eighteen-day old Wistar rats were randomly divided into normal control, model, platelet activating factor (PAF) antagonist prevention and treatment groups. The model of endotoxemia of young rats was established by intraperitoneal injection of endotoxin (5 mg/kg E coli O55: B5 lipopolysaccharide, LPS). The rats in PAF prevention and treatment group were administered with PAF antagonist 0.5 h before and after modeling, respectively. The animals were killed 1.5, 3, 6, 24, 48, and 72 h after LPS injection. The pathological changes of gastric mucosa were observed by hematoxylin-eosin (HE) staining. The content of PGE2 was measured by radioimmunoassay method.

    RESULTS:The pathological changes of gastric mucosa were significant 6 h after LPS injection. Erosion, bleeding and necrosis of gastric mucosa were observed. Swelled epithelial cells and developing degeneration were also observed. However, no remarkable changes occurred in both PAF antagonist groups. In model group, the level of PGE2 in gastric mucosa were significantly lower than that in the control (134.5 ± 9.3 g/L vs 245.1 ± 8.9 ?g/L, P < 0.01) 6 h after LPS injection. In PAF antagonist prevention and treatment groups, the levels of PGE2 were markedly higher than those in the model and control group (304.4 ± 15.0, 315.9 ± 43.7 g/L vs 134.5 ± 9.3, 245.1 ± 8.9 g/L, P < 0.01 or P < 0.05) 6 h after LPS injection.

    CONCLUSION: The level of PGE2 in gastric mucosa is significantly decreased in endotoxemia, but it can be improved by PAF antagonist ......

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