关键词: 结肠直肠肿瘤;转化生长因子-α;寡脱氧核苷酸类;细胞系;脂质体

    摘 要:目的 研究用转化生长因子α(TGF-α)反义脱氧寡核苷酸(ASODN)对人大肠癌细胞株HR8348生长增殖的作用，探讨大肠癌治疗的新途径. 方法 采用人工合成的TGF-α反义及无关对照ODN经阳离子脂质体包裹后转染人大肠癌HR8348细胞.采用MTT法，[3 H]-TdR掺入实验，细胞周期分析，裸鼠体内接种等方法测定瘤细胞体内外增殖的变化. 结果 经TGF-α反义ODN处理的HR8348细胞与对照组HR8348细胞相比，体外增殖速度减慢(细胞增殖抑制率达71%)，DNA合成受抑，裸鼠体内成瘤率下降(25%vs100%). 结论 TGF-α反义ODN能有效抑制大肠癌细胞的体内外生长增殖，可用于实验性肿瘤基因治疗研究.

    Inhibitory effects of TGF┐αantisense oligodeoxynu┐cleotide with liposome on human colorectal cancer cell line

    HE Yong ZHOU Jun WU Jin-Sheng

    1 Department of Hepatobiliary Surgery，Xijing Hos-pital，

    2 Department of Pathology，Stomatological College，Fourth Military Medical University，Xi'an710033，China，

    3 Department of General Surgery，Tangdu Hospital，Fourth Military Medical Univer-sity，Xi'an710038，China

    Keywords:colorectal neoplasms;transforming growth factorα;oligodeoxynucleotides;cell line;liposome

    Abstract:AIM To investigate the effects of TGF-αanti-sense oligodeoxynucleotides(ASODN)on human rectal carci-noma cell line HR8348.METHODS Cationic liposome and lipofectamine were selected as a delivery vector.HR8348cells were treated with ASODN in vitro.HR8348cells with or without pretreatment were inoculated into nude mice.RE┐SULTS Proliferation and DNA synthesis of HR8348cells treated with ASODN were greatly inhibited and tumorigenic rate was greatly reduced.CONCLUSION TGF-αASODN could inhibit the proliferation of human colon cancer cell line in vitro as well as in vivo.The results implicate the potential value in colorectal cancer gene therapy. ......