关键词: 结核菌素衍生蛋白;Fos;免疫应答

    摘 要:目的 进一步阐明中枢神经系统参与免疫调节的部位. 方法 用免疫组织化学方法观察纯化的结核菌素衍生蛋白(PPD)免疫刺激后不同时间点小鼠脑内的Fos蛋白表达. 结果 PPD初次免疫后3，9，14，18和21d时再追加免疫后7，14d的动物，孤束核、延髓腹外侧区、中央灰质、海马、新皮质等多数脑区在各个时间点均可见到较强的Fos表达.但不同时间点的Fos表达不完全相同，尤为明显的是，追加免疫后扣带回出现明显的Fos表达，且左侧较右侧强. 结论 免疫应答过程中有多个脑区参与对免疫信息的感知和调控，皮质在对免疫反应的调节中有侧别差异.

    Fos expression in brain of purified protein deriva┐tive of tuberculin immunized mice

    WANG Ling，HAN Jiang，KUANG Fang，WANG Bai-Ren，JU Gong

    Institute of Neuroscience of Chinese PLA，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:tuberculin purified protein derivative;Fos;im-mune response

    Abstract:AIM To further identify the response of brain ar-eas to the immune stimulus.METHODS Using immunohis-tochemistry method，we observed the Fos expression in the brain of purified protein derivative of tuberculin(PPD)im-munized mice at different time points.RESULTS At3，9，14，18，21d following the primary immunization and7，14d following the second immunization，strong Fos immunostain-ing was observed in many brain areas such as the ventrolater-al medulla ......