关键词: 肝移植;维拉帕米;器官保存;再灌注损伤

    摘 要:目的 在自制的器官保存液XJ-1液已取得较好结果的基础上，探讨维拉帕米对大鼠肝脏低温保存再灌注损伤的防护作用及其机制. 方法 XJ-1液与XJ-1+维拉帕米液保存大鼠肝脏18h后施行肝脏移植，检测术后血清转氨酶水平、肝组织钙离子及丙二醛含量等指标，观察大鼠术后1wk存活率及移植肝脏的组织病理学改变. 结果 XJ-1+维液保存的大鼠肝脏移植后4h，血清ALT，AST水平、肝组织钙离子含量、丙二醛含量均显著低于XJ-1液组(P<0.01，P<0.05)，光镜与电镜下肝组织形态学改变较XJ-1液组好，同时术后生存状况及1wk存活率也优于XJ-1液(71%vs57%). 结论 维拉帕米能够减轻肝脏体外保存再灌注损伤，明显增强了XJ-1液对大鼠肝脏的保存效果，原因在于其阻滞钙通道的钙离子拮抗作用.

    Protective mechanism of verapamil on rat liver grafts under cold preservation and reperfusion

    GAO Chang-Jun ，LI Kai-Zong，GUAN Wen-Xian

    1 Department of Anesthesiology，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China，2 Department of Hepatobiliary Surgery，Xi-jing Hospital，Fourth Military Medical University

    Keywords:liver transplantation;verapamil;organ preserva-tion;reperfusion injury

    Abstract:AIM To investigate the protective effect of vera-pamil on rat liver grafts under cold preservation and reperfu-sion and its mechanism on the basis that we had achieved good effects on rat liver preservation using self-designed XJ-1solution.METHODS Rat orthotopic liver transplants were performed with livers preserved for18h in XJ-1solution and XJ-1+V solution.Serum ALT ......