关键词: 肝炎病毒，乙型;Pres基因;基因;表达;色谱法，亲和

    摘 要:目的 获取乙型肝炎病毒前S区基因(HBVpreS)，序列测定正确后进行融合表达，为今后研究其机制及应用创造条件. 方法 利用乙型肝炎病毒基因组为模板体外扩增HBVPreS基因后进行基因克隆.目的基因经测序正确后克隆入融合表达载体pRSET-C中，转化大肠杆菌JM109(DE3).目的基因经IPTG诱导，由T7启动子调控表达了氨基端带6个连续组氨酸残基的HBV-PreS蛋白，在变性条件下对目的蛋白进行纯化. 结果 成功地扩增到preS区全长基因，得到融合6个His的HBV-PreS蛋白纯度大于90%. 结论 构建了乙型肝炎病毒前S区基因的重组表达载体，获得了稳定表达的工程菌.为以后的深入研究奠定了基础.

    Cloning and fusion expression of HBV┐PreS gene and its purification using affinity chromatography

    YANG Min LIU Xin-Ping HAN Jiong ZHANG Xiao-Guang YAO Li-Bo SU Cheng-Zhi CHENG Chang-Qing

    1 Department of Biochemistry&Molecular Biology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China，

    2 Center of Shanghai Biology Engineering，Academy of Sci-ences of China，Shanghai200032

    Keywords:hepatitis B virus;pres gene;gene;expression;chromatography，affinity

    Abstract:AIM To search the novel protein interacted with HBV-PreS so as to further study its function and mechanism.METHODS Firstly we cloned the gene of PreS.After se-quencing cloned it into the expressing vector pRSET-C ......