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hIL2┐preS融合基因免疫小鼠诱导体液免疫应答
http://www.100md.com 《第四军医大学学报》 2001年第20期
DNA,免疫学,,DNA,免疫学;白细胞介质2;肝炎病毒,乙型,0引言,1材料和方法,2结果,3讨论,参考文献
     关键词: DNA/免疫学;白细胞介质2;肝炎病毒,乙型

    摘 要:目的 探讨HBV preS基因免疫和hIL-2与preS融合基因免疫诱导BALB/c小鼠体液免疫反应的规律. 方法 将preS基因及hIL2-preS融合基因分别克隆入真核表达载体pCMV4,转染COS-7细胞,明确有目的基因表达后,将重组质粒注射BALB/c小鼠臀部肌肉,ELISA方法检测小鼠血清抗体. 结果 pCMV4-preS接种组,3/7小鼠产生抗preS2抗体.pCMV4-hIL2-preS接种组有3/7小鼠产生抗hIL-2抗体,而未检测到抗preS2抗体. 结论 pCMV4-preS可有效诱导小鼠产生抗体反应.hIL2-preS融合基因接种BALB/c小鼠时,可诱导产生抗hIL-2抗体.

    Induction of humoral immune response by hIL┐2┐preS gene immunization of mice

    TANG Li-Xia,MA Wen-Yu,LI Yuan

    Department of Microbiology,Faculty of Preclinical Medicine,Fourth Military Medical University,Xi'an710033,China

    Keywords:DNA/immunology;interleukin-2;hepatitis B virus

    Abstract:AIM To investigate the humoral immune re-sponses to the immunization of mouse BALB/c with the HBV preS gene and the hIL2-preS2fusional gene.METHODS HBV preS gene was cloned into the pUC18-hIL2plasmid,which was connected with the3'terminal of the hIL-2,then the hIL-2-perS fusional gene were inserted into the eukaryot-ic expression vector pCMV4.The pCMV4-hIL2-preS and pCMV4-preS were built and expressed transiently in COS-7cells.After purification ......

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