关键词: 氧;心肌/细胞学;乳酸脱氢酶;丙二醛

    摘 要:目的 观察低浓度(100μmol·L-1 )过氧化氢对心肌细胞的时间损伤效应，探讨在细胞水平建立心肌氧化损伤模型. 方法 胰酶消化法分离培养心肌细胞，随机分为两组:H2 O2 处理组和对照组.在处理后不同的时间点，倒置显微镜下观察心肌细胞每分钟搏动频率;硫代巴比妥酸法检测心肌细胞脂质过氧化代谢产物丙二醛(MDA);生化分析仪检测培养液中乳酸脱氢酶(LDH);台盼蓝排斥试验检测细胞的存活率. 结果 H2 O2 处理后细胞搏动频率先有一个增加过程，而后迅速下降直至停止搏动;细胞MDA的含量随作用时间的延长逐渐升高，30min开始与对照组均差异显著(P<0.01);LDH漏出量也逐渐增加，40min后与对照组均差异显著(P<0.01);H2 O2 处理30min后细胞存活率就开始下降，与对照组相比组间差异显著(P<0.01). 结论 100μmol·L-1 的H2 O2 随着时间的延长表现出明显的细胞损伤效应，可以用它建立模型来研究心肌细胞氧化损伤.

    Experimental model of H2 O 2 ┐induced oxidative in┐jury in cardiomyocytes

    ZHENG Yan-Song，LI Yuan，ZHANG Shan-Hong，GONG Wei-Qin，SUN Bin

    Department of Geriatrics，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:oxygen;myocardium/cytology;lactate dehydro-genase;malondialdehyde

    Abstract:AIM To investigate the time-course of H2 O2 -in-duced injury in cardiomyocytes and establish an experimental model of oxidative injury by using cultured neonatal rat ven-tricular myocytes.METHODS Ventricular myocytes were isolated from neonatal mice and cultured for4～6days.Cells were divided into2groups randomly:control group and H2 O2 (100μmol·L-1 )group.After cells were exposed to H2 O 2 ......