Keywords:vascular endothelial cell;vascular smooth muscle cell;proliferation

    Abstract:AIM To study the effects of vascular endothelial cell conditioned medium(ECCM)，endothelin-1(ET-1)，en-dothelin-converting enzyme inhibitor phosphoramidon on the proliferation of vascular smooth muscle cell(SMC)and vas-cular smooth muscle cell conditioned medium(SMCCM)on the proliferation of vascular endothelial cell(EC)in vitro.METHODS EC and SMC were isolated from rabbit aortas and cultured.The conditioned medium(CM)of EC and SMC were harvested.ECCM，ECCM conditioned in the presence of phosphoramidon，SMCCM and ET-1were added individu-ally to the cultures.Proliferation rate of the cells was mea-sured with3H-thymidine incorporation method.RESULTS ECCM and ET-1significantly increased the proliferation of SMC in a dose-dependent manner with a maximal effect of(190±11)%(100%ECCM)and(166±9)%(100ng·L-1 ET-1)respectively.ECCM conditioned in the presence of phosphoramidon reduced the mitogenic effect by(33±2)%.SMCCM elicited a dose dependent decrease of cultured EC proliferation with a maximal effect of(78±3)%over basal level. ......