Keywords:formaldehyde;genes，FOS;astrocytes;glial fib-rillary acidic protein;immunohistochemistry CLC number:R322.81 Document code:A

    Abstract:AIM To observe the response of astrocytes and neurons to noxious stimulation from the upper lip.METH┐ODS Formalin was subcutaneously injected into the upper lip unilaterally.The distribution and relationship between the reactive neurons and astrocytes in the rat brain were studied at1，3and6h later with a triple labelling method combining GFAP，Fos and TH immunohistochemical staining.RE┐SULTS ①FOS Positive neurons and glial fibrillary acidic protein(GFAP)positive astrocytes were specifically dis-tributed over the functional area involved with the transmis-sion and relief of pain sensation;②Reactive neurons contact-ed the reactive astrocytes and formed3kinds of neuron-astro-cytes complex(NASC)--TH+/FOS+/GFAP+triple la-belled，TH+/FOS+/GFAP-double labeled，and FOS+/GFAP+/TH-double labelled NASC.CONCLUSION Neu-ronal-astrocytic complex may play an important role in pro-cessing signals of noxious stimulation from the lip.

    大鼠上唇皮下注射Formalin后脑内星形胶质细胞的反应及其与神经元的关系┐光镜研究 ......