关键词: 卵巢肿瘤;细胞系;端粒酶;逆转录酶抑制剂

    摘 要:目的 研究逆转录酶抑制剂(AZT)对卵巢肿瘤细胞端粒酶活性的影响. 方法 采用TRAP-ELISA方法检测卵巢癌HO-8910细胞在AZT作用前后端粒酶活性的变化，以流式细胞仪分析细胞周期的改变. 结果 AZT作用后的HO-8910细胞端粒酶活性明显受抑制，且细胞的G2 /M期DNA含量明显增高. 结论 AZT能明显抑制肿瘤细胞的端粒酶活性.

    AZT inhibits telomerase activity of tumor cells

    LI Hong-Mei，XIN Xiao-Yan，DU Hui，WANG Jian，WANG De-Tang

    Department of Obstetrics and Gynecology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:ovarian neoplasms;cell line;telomerase;reverse transcriptase inhibitor

    Abstract:AIM To study the effect of inhibition of telom-erase activity by reverse transcriptase inhibitors(AZT)on o-varian cancer cells in vitro.METHODS Telomerase activity was determined by TRAP-ELISA in untreated and treated HO-8910cells by AZT，cell cycle phases were analyzed by flow-cytometry.RESULTS Telomerase activity of HO-8910cells was significantly inhibited when treated with AZT ......