关键词: 激光;大鼠;脊髓;降钙素基因相关肽

    摘 要:目的 研究10mW氦氖(He-Ne)激光照射对大鼠坐骨损伤神经后脊髓CGRP表达的影响. 方法 96只SD大鼠制成坐骨神经损伤模型，He-Ne激光照射损伤的坐骨神经，免疫组化方法观察脊髓内CGRP的变化. 结果 神经损伤后1d脊髓内CGRP表达即增加;2wk及4wk时激光照射组CGRP的表达高于对照组，阳性表达主要存在于脊髓灰质内前角运动神经元和背根感觉纤维;8wk时CGRP仍保持较高水平的表达，二组无明显差异. 结论 10mW He-Ne激光照射大鼠损伤的坐骨神经可引起脊髓内神经元CGRP表达的变化，有促进完成神经再生的作用.

    Effect of He┐Ne laser irradiation on CGRP expres┐sion in rat spinal cord after nerve injury

    WANG Bing-Shui YI Nan LI Ling MA Hong WANG Jian-Bo WANG Hong

    1 Department of Physiotherapy&Rehabilitation，

    2 Department of Pathology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:laser;rat;spinal cord;calcitonin gene-related peptide

    Abstract:AIM To study the expression of calcitonin gene-related peptide(CGRP)in spinal cord after irradiation on in-jured sciatic nerve of rat by He-Ne laser with output power of10mW.METHODS Injured sciatic nerves，made in96SD rats，were irradiated by He-Ne laser.The changes of CGRP in spinal cord were immunohistochemically studied.RE┐SULTS Expression of CGRP increased in spinal cord on the first day after injury of sciatic nerve.More expression of CGRP in second and fourth week was observed in laser irradi-ation group compared to control group.The positive sub-stance was mainly found in motor neuron in anterior horn and sensory nerve fiber in posterior horn.At eighth week ......