关键词: 神经元;烟碱;受体，胆碱能;抗体;重症肌无力

    摘 要:目的 用重症肌无力(MG)患者的乙酰胆碱受体抗体(AChRab)选择性地显示神经元烟碱型乙酰胆碱受体. 方法 用Elisa法测定MG患者血中的AChRab滴度，然后用硫酸铵盐析和免疫亲和层析法提取纯化AChRab，再用免疫组化法探讨AChRab与大鼠CNS神经元-nAChR之间的免疫结合反应. 结果 首次表明AChRab与神经-nAChR之间的阳性免疫结合反应广泛分布于鼠CNS许多部位，如大脑皮层、脑干各颅神经运动核团、脊髓运动神经元等部位，与用其他方法显示者其分布一致或类同. 结论 MG患者AChRab可与大鼠和猴CNS神经-nAChR交叉结合;可用于选择性地显示CNS神经-nAChR.

    Novel method of expression of central neuronal nicotinic acetylcholine receptor

    LI Zhu-Yi，QIU Xiao-Fei

    Department of Neurology，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China

    Keywords:neurons;nicotine;receptors，cholinergic;anti-bodies;myasthenia gravis

    Abstract:AIM To study the method of expression of neu-ronal nicotinic acetylcholine receptor by acetylcholine receptor antibody purified from myasthenia gravis serum.METHODS ELISA assay was applied to detect the acetylcholine recep-tor antibody(AChRab).AChRab was purified by im-munoaffinity tomography method and was used to study the immunoreaction between acetylcholine receptor antibody and rat central nervous system neuronal nicotinic acetylcholine re┐ceptor.RESULTS The positive immunoreaction between acetylcholine receptor antibody and central neuronal nicotinic acetylcholine receptor was widely distributed in rat central nervous system ......