摘 要: 目的: 验证日本血吸虫基因重组抗原检测水牛日本血吸虫病的效果。 方法: 以日本血吸虫基因重组抗原 LHD-Sj23/pGEX和SjCL/pET-28a(+)作为抗原，应用ELISA法检测水牛日本血吸虫病。结果: 感染日本血吸虫病 阳性符合率分别为 93.3% (42/45)和 95.6% (43/45)，阴性符合率分别为 93.3% (42/45)和80%(36/45)。和以血 吸虫成虫抗原(SWAP)和虫卵(SEA)抗原作为诊断抗原获得的结果差异不明显。同时将上述两种基因重组抗原混 合作为诊断抗原，检测水牛血吸虫病，结果阳性符合率为88.9% (40/45)，阴性符合率为33.3% (15/45)。结论: 由于基因重组抗原制备简便、价廉，有利于诊断技术的标准化。因此，基因重组抗原在血吸虫病诊断领域有着良好的 研究和应用前景。

    关键词: 基因重组抗原;日本血吸虫病;诊断

    DIAGNOSING OF WATER BUFFALO SCHISTOSOMIASIS JAPONICA BY USING Sj23 and SjCL RECOMBINANT ANTIGENS

    LU Ke， LI Hao， SHI Yao-jun， LIN Jiao-jiao

    (Shanghai Institute of Domestic Animal Parasitology， CAAS; Key Laboratory of Animal Parasitology Ministry of Agriculture， Shanghai 200232， China)

    Abstract: The fusion protein LHD-Sj23/pGEX and SjCL/pET-28a(+)were employed as diagnosing anti- gen for detecting water buffalo Schistosomiasis japonica by ELISA. The result showed that95.6% (43/45) and93.3% (42/45) positive coincidence rates， 93.3% (42/45) and 80%(36/45) negative coincidence rates were obtained from buffalo. The similar results were obtained by using SWAP and SEA as diagnosing antigen comparing with thatof recombinant LHD-Sj23/pGEX and SjCL/pET-28a(+). At the same time ......