关键词: 汉坦病毒;S基因;疫苗，DNA;基因表达;抗体生成

    摘 要:目的 在本室前期工作的基础上，进一步进行汉坦病毒S基因0.7kb片段的真核表达及基因免疫的研究. 方法 构建汉坦病毒76-118株S基因5'端700bp片段的真核表达载体pcDNA3.1-S0.7，脂质体法转染COS-7细胞，免疫荧光检测表达结果;用该质粒免疫BALB/c小鼠，并用免疫荧光法及ELISA法检测基因免疫的体液免疫应答效果. 结果 免疫荧光检测结果表明，目的基因在COS-7细胞中获得瞬时表达;用pcDNA3.1-S0.7基因免疫小鼠可引起抗汉坦病毒核蛋白(NP)特异性的抗体应答. 结论 汉坦病毒S基因0.7kb片段可刺激机体产生特异的抗汉坦病毒体液免疫应答，为进一步进行细胞免疫反应的检测及基因疫苗的研究提供实验基础.

    Construction，expression and gene immunization of eukaryotic expression vector containing0.7kb frag┐ment of Hantaan virus S segment

    ZHANG Fang-Lin，XU Zhi-Kai，YAN Yan，BAI Wen-Tao，WU Xing-An，LUO Wen，ZHAO Qian Department of Microbiology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:Hantaan virus;S segment;vaccines，DNA;gene expression;antibody formation

    Abstract:AIM To investigate the expression and immuno-genicity of recombinant eukaryotic plasmid containing compe-tent fragment of Hantaan virus S segment.METHODS Re-combinant eukaryotic expression vector pcDNA3.1-S0.7was constructed by cloning S segment0.7kb fragment into pcDNA3.1(+)and transfected COS-7cells by lipofectin.Immunoflourescence was used to identify the expression.Af-ter BALB/c mice were vaccinated by pcDNA3.1-S0.7 ......