关键词: 肿瘤坏死因子-α;白细胞介素-6;卵泡液;雌激素;卵母细胞

    摘 要:目的 测定卵泡液中肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平，并探讨其与雌二醇(E2 )产生及卵母细胞成熟度的关系. 方法 酶联免疫法测定97份卵胞液及26份血清标本的TNF-α和IL-6水平，放射免疫法测定E2 水平，并记录每份卵泡液中卵母细胞的成熟度. 结果 卵泡液中TNF-α和IL-6水平分别为(26.5±7.2)ng L-1 和(328.0±48.7)ng L-1 ，分别较血清中TNF-α和IL-6的水平高9倍和20倍(P<0.01);卵泡液TNF-α和IL-6水平与卵泡液中E2 水平及卵母细胞成熟度呈正相关(r值为0.648和0.692，P<0.01). 结论 卵泡液中存在较高水平的TNF-α和IL-6，其与E2 产生及卵母细胞成熟度有一定关系.

    Concentrations of TNF┐αand IL┐6in follicular flu┐id and their influence on ovarian function

    ZHAO Hai-Bo LIU Yi

    1 Department of Obstetrics and Gynecology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Obstetrics and Gynecology，Tongji Medical University

    Keywords:tumor necrosis factor-α;interleukin-6;follicular fluid;estrogen;oocyte

    Abstract:AIM To detect the concentrations of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in human follicular fluid and to evaluate their relationships to estradiol production and oocyte maturity.METHODS TNF-αand IL-6concentrations in97samples of follicular fluid and26serum samples from26patients undergoing IVF-ET were measured with enzyme-linked immunosorbent assay ......