摘 要:目的 为比较对猪囊尾蚴总RNA提取与mRNA分离方法的优点。 方法 采用Promega公司的SV总RNA分离体系、RNAgents总RNA分离体系和BioDev-Tech的TRIZOL LS试剂，对猪囊尾蚴总RNA进行了提取;用Promega的PolyATtract mRNA分离体系和Amersham的Quickprep微量mRNA纯化试剂盒对mRNA进行分离。 结果 表明经改良的TRIZOL LS试剂，提取猪囊尾蚴RNA效果好，而PolyATtract mRNA分离体系分离的mRNA含量高，纯度好。 结论 所分离的mRNA适合于构建cDNA表达文库。

    关键词: 猪囊尾蚴;RNA提取;mRNA分离

    TOTAL RNA EXTRACTED AND mRNA ISOLATED FROM CYSTICERCUS CELLULOSAE

    ZONG Rui-qian， JING Zhi-zhong， CAI Xue-peng

    (1.Life school of Xiamen University，Xiamen，361005，China;2.Lanzhou Veterinary Research Institute of CAAS，Lanzhou，730046，China;3.Veterinary Department of Gansu Agricultural University，Lanzhou，730070，China)

    Abstract: Total RNA was extracted and mRNA was isolated from Cysticercus cellulosae with different methods.It was proved that TRIZOL LS agents is suitable for the total RNA extracted and PolyATtract mRNA isolation system is better for the mRNA isolated.

    Key words: Cysticercus cellulosae RNA;mRNA

    猪囊尾蚴是寄生于人体肠道的有钩绦虫的幼虫期 ......