关键词: 角蛋白;自身抗体;表皮/生长和发育

    摘 要:目的 观察抗角蛋白自身抗体(anti-keratin auto an-tibodies，AK auto Ab)对体外表皮形成的影响，研究AK auto Ab在表皮自身稳定中的作用. 方法 采用贴壁法体外培养皮肤，比较观察不同浓度AK auto Ab对表皮形成速率的影响，并采用Brd-U掺入法测定各组细胞增殖率. 结果 在游走期，AK auto Ab可以促进表皮形成速率，并呈剂量效应关系;各处理组间角质形成细胞增殖率无差异. 结论 AK auto Ab具有促进游走期表皮形成的作用，这可能与其促进角质形成细胞游走有关.

    Effects of AK auto Ab on epidermazation in vitro

    LI Wei，LIU Yu-Feng，LI Zhi-Qiang，GAO Tian-Wen

    Center of Dermatology&Venereal Diseases of Chi-nese PLA，Xijing Hospital，Fourth Military Medi-cal University，Xi'an710033，China

    Keywords:keratin;autoantibodies;epidermis/growth&de-velopment

    Abstract:AIM To investigate the effects of antikeratin au-toantibodies(AK auto Ab)on epidermazation in organ cul-ture of skin，and to make further studies on the significance of AK auto Ab in skin homeostasis.METHODS AK auto Ab was purified from pooled serum of healthy human by us-ing keratin affinity chromatography.Epidermal explant cul-ture was applied ......