复方二精灵__(药理药化)

复方二精灵多糖抗脑缺血缺氧损伤的分子机理

http://www.100md.com 《时珍国医国药》 2005年第11期

复方二精灵,,复方二精灵；海马神经元；电压门控性钠通道,1材料与方法,2结果,3讨论,参考文献

     摘要：目的：研究复方二精灵多糖抗脑缺血损伤的分子机理。方法：采用全细胞膜片钳技术记录急性分离的小鼠海马CA1区神经元电压门控性钠电流，观察复方二精灵多糖对钠电流的影响，拟从离子通道水平揭示复方二精灵的抗缺血缺氧脑损伤作用。结果：0.1%复方二精灵多糖显著抑制海马神经元电压门控性钠通道电流锋值，使钠电流的半激活电压向去极化方向偏移，不影响钠电流的稳态失活及复活曲线。结论：复方二精灵多糖对海马神经元电压门控性钠电流的抑制作用可能为复方二精灵抗缺血缺氧脑损伤的重要机理。

    关键词：复方二精灵；海马神经元；电压门控性钠通道

    The Molecular Mechanism of Compound Erjingling Polysaccharide Neuroprotection Effect on Hypoxia and Ischemia Damage

    YIN Shijin，LIU Xiangming，WU Qunrong，MEI Zhinan

    (1.The Biomedical Engineering Institute of Centralsouth University for Nationalities,Wuhan 430074, China; 2.Institute of Nationality Materia Medica, Central-south University for Nationalities, Wuhan 430074, China)

    Abstract:Using wholecell patch clamp technology on the membrane of freshly isolated hippocampal neurons , the effects of Compound Erjingling polysacharide on voltagegated sodium currents were observed to investigate its molecular mechanism of neuroprotection in hypoxia and ischemia damage. The experimental data demonstrated that peak sodium currents of hippocampal neurons decreased significantly after application of 0.1% Compound Erjingling polysaccharide (from 100% to 60.96± 12.13%, n=16 ......

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