【摘要】 目的 探讨载脂蛋白E(apoE)基因多态性与轻度认知障碍(MCI)的关系。方法 应用限制性片段长度多态分析-聚合酶链反应(RFLP-PCR)方法，测定56例MCI患者和89例同龄对照组apoE基因型和等位基因频率。结果 MCI组ε2、ε3、ε4等位基因频率分别为7.14%、71.38%和21.48%，而同龄对照组分别为7.30%、84.83%和7.87%。apoEε4等位基因在MCI患者中出现的频率明显高于对照组，统计学分析说明差异具有非常显著性(P<0.01)。结论 apoEε4等位基因是MCI的危险因素。

    【关键词】 轻度认知障碍；多态性；载脂蛋白E；基因型

    An association analysis of apolipoprotein E genotypes with mild cognitive impairment

    YE Ni,ZHANG Lin-hong.

    Department of Neurology,The Central Hospital of Wuhan，Wuhan 430014，China

    【Abstract】 Objective To analyse the association of apolipoprotein E(apoE) genotypes with mild cognitive impairment(MCI).Methods RFLP-PCR technology is applied to analyse the apoE allele frequency of 56 MCI patients and 89 controls.Results Among the 56 cases of MCI,the frequency of apoEε2,ε3,ε4 are 7.14%,71.38%,21.48% respectively,while in the 89 controls they are 7.30%，84.83%，7.87% respectively.The frequencies of the two groups have significant difference(P<0.01)and the frequency of apoEε4 allele is higher in MCI than that in the controls and the statistical treatment suggest that there is significant difference(P<0.01).Conclusion The apoEε4 allele is a genetic risk factor of MCI. ......