关键词: 大鼠;四氯化碳;肝/损伤;NG-硝基精氨酸甲酯

    摘 要:目的 研究一氧化氮在大鼠慢性中毒性肝损伤中的作用. 方法 制备慢性CCl4 中毒性肝损伤大鼠模型12wk，肝损伤模型大鼠随机分为NO合酶(NOS)抑制剂治疗组和未治疗组，治疗组用L-NAME0.5mg·kg-1 ·d-1 ，胃内注入;肝损伤组和正常对照组用生理盐水灌胃，每日1次，每组均为10d.镉还原比色法测定各组血清中NO含量;测定各组血清中ALT，AST和TBil，行肝组织学检查. 结果 肝损伤组鼠血清NO含量明显高于正常对照组鼠和L-NAME治疗组[(8.2±5.5)μmol·L-1 ，(5.7±3.6)μmol·L-1 ，(5.9±6.1)μmol·L-1 ，P<0.01].血清ALT和AST含量亦呈同样改变，有显著性差异(P<0.01).肝组织学检查显示L-NAME治疗组大鼠肝组织炎症明显减轻. 结论 L-NAME对大鼠慢性CCl4 中毒性肝损伤具有的保护作用.

    Protective effect of L┐NAME on chronic liver injury induced by CCl 4 in rats

    GUO Xue-Gang，MIAO Ji-Yan，WANG Xin，ZHANG Zong-You

    Chinese PLA Institute of Gastroenterology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:rats;carbon tetrachloride;liver/injuries;NG-ni-troarginine methyl ester

    Abstract:AIM To investigate the effect of NO on chronic liver injury induced by carbon tetrachloride(CCl4 )in rats.METHODS Rats with cirrhosis induced by carbon tetrachlo-ride were randomly separated into two groups，one receiving0.5mg·Kg-1 ·d-1 per day of NG -nitro-L-arginine methyl ester(L-NAME)during10d ......