关键词: 心肌细胞;凋亡;羟自由基

    摘 要:目的 观察外源性羟自由基对心肌细胞凋亡的诱导作用，并探讨其可能的信号转导通路. 方法 ①利用培养的第2代心肌细胞，随机分成4组，在终浓度为10-4 mol L-1 ，10-3 mol L-1 的过氧化氢[一组加半胱天冬酶-3(caspase-3)的抑制剂(DEVD-FMK)10μmol L-1 ]无血清条件下培养24h.观察各组心肌细胞存活率，TUNEL法检测凋亡，透射电镜观察细胞超微结构，流式细胞仪检测细胞的凋亡率. 结果 ①外源性羟自由基对心肌细胞生长具有明显抑制作用.②低浓度过氧化氢(100μmol L-1 )可诱导培养的心肌细胞发生凋亡，心肌细胞呈现特征性超微结构变化，TUNEL检测呈现黄绿色荧光，流式细胞仪PI染色法呈现典型的亚二倍体峰.高浓度时则主要致使心肌细胞坏死.③caspase-3抑制剂(DE-VD-FMK)可阻断外源性OH-对心肌细胞生长的抑制作用，降低心肌细胞的凋亡率. 结论 低浓度羟自由基能诱导心肌细胞凋亡，其信号转导通路包括caspase-3途径.

    Apoptosis of neonatal rat cardiomyocytes induced by exogenous hydroxyl free radical and the effect of caspase┐3antagonist

    CHENG He-Xiang，ZHANG Rong-Qing，MA Ying-Yan，JIA Guo-Liang，LI Fei，HU Tao，RU Lei-Sheng

    1 Department of Cardiovasology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China，2 Department of Cardiovasology，General Hospital of Shenyang Military District，Shenyang110015，China

    Keywords:cardiomyocytes;apoptosis;hydroxyl free radical

    Abstract:AIM To observe the effect of exogenous hydroxyl free radical on apoptosis of neonatal rat cardiomyocytes(NRCs)and to investigate its possible mechanism.METH┐ODS ①The second passage of NRCs were randomly divided into four groups and were cultured with hydrogen peroxide(10 -4 mol L-1 ......