热休克蛋白70__(研究原著)

HSP70诱饵载体的构建及其在酵母双杂交系统中自激活作用的检测

http://www.100md.com 《第四军医大学学报》 2003年第5期

热休克蛋白70,,热休克蛋白70；酵母双杂交系统；自激活作用,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Recombination of bait vector pGBKT7Hsp70 and the test of its autonomous activation in the yeast two hybrid system

    LI Jing, YANG ShouJing, LIU YanFang, ZHOU Peng, HAN Hua

    Department of Pathology, Department of Medical Genetics and Developmental Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To construct the bait vector (pGBKT7Hsp70) of MATHMAKER GAL4 TwoHybrid System 3, and test it for its autonomous activation. METHODS: Hsp70 gene was amplified by PCR from pBBS212Hsp70 plasmid, and the EcoRI and BamHI sites were introduced into it. The Hsp70 gene, after sequenced, was cloned into pGBKT7. Selfactivation of the recombination vector (pGBKT7Hsp70) was tested. RESULTS: The pGBKT7Hsp70 vector was successfully constructed and had no autonomous activation. CONCLUSION: The pGBKT7Hsp70 was proved to be suitable for further research on the genes in tumors, which interact with the Hsp70.

    【Keywords】 Hsp70; yeast two hybrid system; autonomous activation

    【摘要】目的：构建用于酵母双杂交系统的“诱饵”载体pGBKT7Hsp70 ......

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