Effect of Shenkang injection on proliferation of glomerular mesangial cell

    LI Feng, WANG Hanª²Min, HANG Li, WANG Changª²Hai, CHENG Qingª²Li,YE Chuanª²Hui

    Department of Traditional Chinese Mendicine, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, Department of Nephrology,General Hospital of Chinese PLA, Beijing 100853, China, Department of Nephrology, Affiliating Hospital,Chengdou University of Traditional Chinese Mendicine, Chengdou 610075, China

    ¡¾Abstract¡¿ AIM: To explore the mechanism of ShenKang Injection (SKI) on prohibiting glomerulosclerosis (GS) so as to provide fresh evidence for clinical application of SKI in treating chronic renal failure (CRF). METHODS: Using serumª²pharmacological test of Chinese herbs, the effects of SKI on the proliferation in cultured human glomerular mesangial cells (GMC) and the level of fibronectin (FN) secretion in cultured GMC were observed. RESULTS: SKI and serum with SKI significantly inhibited the proliferation and the level of secreting FN in cultured GMC in a concentrationª²dependent manner (P<0.01, P<0.05). With the prolonging of stimulating time, the inhibitory effects SKI increased on the proliferation and the level of FN, but the effects of serum with SKI decreased on the proliferation and the level of FN. CONCLUSION: The effects of SKI on GMC differ from those of serum with SKI. It is indicated that the effective mechanism of SKI is complicated and is related to SKI metabolism. ......