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一氧化氮对急性肺损伤发病过程中核因子-kB活化的调节作用
http://www.100md.com 李海龙, 陈海龙, 张 波, 贺雪梅, 徐 宏,
核因子-kB;一氧化氮;急性肺损伤;急性重症胰腺炎;诱生性一氧化氮合酶李海龙,陈海龙,张波,贺雪梅,徐宏,范奇.一氧化氮对急性肺损伤发病过程中核因子-kB活化的调节作用. 世界华人消化杂志
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     李海龙,陈海龙, 大连医科大学附属第一医院普外科 辽宁省大连市 116011

    张波,徐宏, 范奇,大连生物技术研究所 辽宁省大连市 116027

    贺雪梅,大连医科大学中山学院生理教研室 辽宁省大连市 116023

    李海龙, 男, 1976-09-25生, 辽宁省海城市人, 汉族, 大连医科大学中西医结合专业博士, 主要从事全身炎症反应综合征发病过程中有关分子机制方面的研究.

    国家自然科学基金资助项目, No.30271667

    通讯作者: 陈海龙, 116011, 辽宁省大连市, 大连医科大学附属第一医院普外科. hailong_chen@x263.net

    电话:0411-83600680 传真: 0411-83600680

    收稿日期: 2005-09-10 接受日期: 2005-09-30

    Regulatory effects of nitric oxide on nuclear factor-kappa B activation during acute lung injury

    Hai-Long Li, Hai-Long Chen, Bo Zhang, Xue-Mei He, Hong Xu, Qi Fan

    Hai-Long Li, Hai-Long Chen, Department of Surgery, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China

    Bo Zhang, Hong Xu, Qi Fan, Dalian Institute of Biotechnology, Dalian 116027, Liaoning Province, China

    Xue-Mei He, Department of Physiology, Dalian Medical University, Dalian 116027, Liaoning Province, China

    Supported by National Natural Science Foundation of China, No.30271667

    Correspondence to: Dr. Hai-Long Chen, Department of Surgery, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province,

    China. hailong_chen@x263.net

    Received: 2005-09-10 Accepted: 2005-09-30

    Abstract

    AIM: To further present the double-function of nitric oxide (NO) on acute lung injury (ALI) induced by severe acute pancreatitis (SAP) as well as its relation with the activation of nuclear factor kappa B (NF-kB).

    METHODS: Sprague Dawley rats were averagely divided into five groups (6 for each): sham operation (control), model, sodium nitroprussi-de (SNP), L-Arg, and aminoguanidine (AG) treatment group. Sodium deoxycholate (15 g/L) was injected retrogradely into the common biliopancreatic duct of rats to establish the model of SAP. Meanwhile, in vitro stimulation experiment (using lipopolysaccharide, LPS) was also performed. The activity of NF-kB and the amount of inducible nitric oxide synthase (iNOS) mRNA in alveolar macrophages were determined by electrophoretic mobility shift assay and reverse transcription polymerase chain reaction (RT-PCR), then the levels of tumor necrosis factor a (TNF-a), NO and iNOS expression were also measured respectively.

    

    RESULTS: The levels of NF-kB, iNOS mRNA, TNF-a, NO, iNOS in alveolar macrophages and serum of model group were significantly higher than those in control group (P = 0.02). Other indexes about ALI and pathological examination of lung tissue showed obvious pathogenic changes in model group. SNP and AG decreased the NF-kB activity (213.47 ± 12.34, 222.98 ± 17.69 vs 327.13 ± 13.46, P < 0.05), reduced iNOS mRNA expression (SNP: 2.35 ± 0.34 vs 3.1 ± 0.38, P < 0.05), TNF-a (0.38 ± 0.034, 0.45 ± 0.043 mg/L vs 0.76 ± 0.045 mg/L) and NO (168.2 ± 0.78, 146.4 ± 0.59 mmol/L vs 229.3 ± 0.98 mmol/L) level and alleviated the degree of lung injury, but no significant effect was shown in L-arg group ......

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