RT-PCR 寻找大肠癌患者外周血早期分子标记物的研究
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杜雅菊, 刘晓珺, 金英敏, 曲 波, 李宝杰
RT-PCR;分子标记物;早期诊断;外周血;大肠癌杜雅菊,刘晓珺,金英敏,曲波,李宝杰.RT£PCR寻找大肠癌患者外周血早期分子标记物的研究. 世界华人消化杂志200
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杜雅菊,刘晓珺,金英敏,曲波, 李宝杰, 哈尔滨医科大学附属二院消化科 黑龙½省哈尔滨市 150086
杜雅菊,女, 1964-09-25生,黑龙½省哈尔滨市人, 汉族, 2003年哈尔滨医科大学消化专业博士,副主任医师, 主要从事肿瘤临床及基础研究.
黑龙江省科学技术厅攻关项目, No. GB02C146-01
通讯作者: 杜雅菊, 150086, 黑龙½省哈尔滨市学府路246号, 哈尔滨医科大学附属二院消化科. duyaju@medmail.com.cn
电话: 0451-86605143 89576751
收稿日期:2005-11-15 接受日期: 2005-11-24
Investigation of molecular tumor markers in peripheral blood for early diagnosis of large intestinal carcinoma patients using RT-PCR technique
Ya-Ju Du, Xiao-Jun Liu, Ying-Min Jin, Bo Qu, Bao-Jie Li
Ya-Ju Du, Xiao-Jun Liu, Ying-Min Jin, Bo Qu, Bao-Jie Li, Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Supported by the Project of Science and Technology Department of Heilongjiang Province, No. GB02C146-01
Correspondence to: Ya-Ju Du, Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Harbin 150086, Heilongjiang Province, China. duyaju@medmail.com.cn
Received: 2005-11-15 Accepted: 2005-11-24
Abstract
AIM: To search for molecular tumor markers in the peripheral blood from patient with early stage of large intestinal carcinoma, and to offer early, reliable and simple methods for the early diagnosis of large intestinal carcinoma.
METHODS: The expression of CK-20, GST-π, hTERT, survivin and skp2 mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR) in the peripheral blood from patients with large intestinal carcinoma (n = 28), adenoma accompanied by moderate and severe atypical hyperplasia (n = 8), adenoma accompanied by light atypical hyperplasia (n = 18), and inflammatory polypus (n = 11) as well as normal controls (n = 10).
RESULTS: The positive rate of hTERT mRNA expression was 82.1%(23/28), 87.5%(7/8), 27.8%(5/11), 9 ......
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