Ss-A/Ro 核蛋白 60 ku 亚单位在胃癌多药耐药中的作用
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韩全利, 丁 杰, 张龙方, 王 新, 郭长存,
胃癌;Ss-A,Ro60ku亚单位;多药耐药;基因转染韩全利,丁杰,张龙方,王新,郭长存,乔泰东,张学庸,樊代明.Ss-A,Ro核蛋白60ku亚单位在胃癌多药耐药中的作用. 世界华人消化杂
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韩全利,张龙方, 空军总医院干部病房三区 北京市 100036
丁杰,王新, 郭长存, 乔泰东,张学庸, 樊代明, 第四军医大学西京医院全军消化病研究所 陕西省西安市 710032
韩全利,男, 1971-06-24生,山西省文水县人, 汉族, 2003年第四军医大学博士毕业,主要从事胃癌多药耐药的研究.
国家自然科学基金资助项目, No. 30371623
通讯作者: 丁杰, 710032, 陕西省西安市长乐西路15号, 第四军医大学西京医院全军消化病研究所. dingjie@fmmu.edu.cn
电话: 029-83375230 传真: 029-82539041
收稿日期:2005-11-16 接受日期:2005-12-20
Function of Ss-A/Ro ribonucleoprotein 60-ku subunit in multi-drug resistance of gastric cancer
Quan-Li Han, Jie Ding, Long-Fang Zhang, Xin Wang, Chang-Cun Guo, Tai-Dong Qiao, Xue-Yong Zhang,
Dai-Ming Fan
Quan-Li Han, Long-Fang Zhang, Department of Geratology, General Hospital of Air Force of Chinese PLA, Beijing 100036, China
Jie Ding, Xin Wang, Chang-Cun Guo, Tai-Dong Qiao, Xue-Yong Zhang, Dai-Ming Fan,Department of Gastroenterology, Xijing Hospital, the Fourth Military Medical University, Xian 710032, Shaanxi Province, China
Supported by National Natural Science Foundation of China, No. 30371623
Correspondence to: Dr. Jie Ding, Department of Gastroenterology, Xijing Hospital, the Fourth Military Medical University, Xian 710032, Shaanxi Province,
China. dingjie@fmmu.edu.cn
Received: 2005-11-16 Accepted: 2005-12-20
Abstract
AIM:To investigate the possible function of Ss-A/Ro ribonucleoprotein 60-ku subunit (Ro 60) in the multi-drug resistance (MDR) of gastric cancer cell line.
METHODS: The Ro 60 encoding gene was cloned using reverse transcription polymerase chain reaction (RT-PCR). DNA recombination technique was used to construct Ro 60 sense eukaryotic expression vector, and then the product was transfected into SGC7901 cells by LipofectamineTM2000. Drug susceptibility in the chemotherapy was analyzed by MTT assay, and IC50 value of gastric cancer cells to chemotherapy drugs was calculated. The intracellular accumulation of adriamycin in gastric cancer cells was measured using fluorescence-activated cell sorting ......
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